A D-cysteine desulfhydrase, SlDCD2, participates in tomato fruit ripening by modulating ROS homoeostasis and ethylene biosynthesis.
A D-cysteine desulfhydrase, SlDCD2, participates in tomato fruit ripening by modulating ROS homoeostasis and ethylene biosynthesis.
复制标题
D-半胱氨酸脱硫酶SLDCD2通过调节ROS同种异体和乙烯生物合成来参与番茄果实成熟。
DOI：
10.1093/hr/uhad014
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发表时间：
2023-03
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
作者：
硫化氢（H2S）参与植物生长和发育过程中的多个过程。 D-半胱氨酸脱硫酶（DCD）能以D-半胱氨酸为底物产生H2S；然而，DCD 在番茄生命周期中的潜在发育作用尚未得到探索。在本研究中，SlDCD2 在果实成熟过程中表现出表达增加。与对照果实相比，pTRV2-SlDCD2沉默SlDCD2加速了果实成熟。通过CRISPR/Cas9转化构建了SlDCD2基因编辑突变体，该突变体表现出加速果实成熟、减少H2S释放、更高的总半胱氨酸和乙烯含量、增强叶绿素降解和增加类胡萝卜素积累。此外，多个成熟相关基因（包括NYC1、PAO、SGR1、PDS、PSY1、ACO1、ACS2、E4、CEL2和EXP）的表达在dcd2突变体番茄果实成熟过程中得到增强。与野生型果实相比，SlDCD2突变导致果实中H2O2和丙二醛（MDA）积累，导致活性氧（ROS）代谢失衡。相关分析表明，H2O2含量与类胡萝卜素含量、乙烯含量、成熟相关基因表达量呈强正相关，与叶绿素含量呈负相关。此外，dcd2 突变体表现出较早的叶片衰老，这可能是由于 ROS 稳态受到干扰。简而言之，我们的研究结果表明，SlDCD2 参与 H2S 的产生，并且 dcd2 突变体中内源 H2S 产生的减少会导致果实加速成熟和叶片过早衰老。此外，dcd2 突变体中 H2S 的减少会导致 H2O2 过量积累和乙烯释放增加，这表明 H2S 和 SlDCD2 在调节 ROS 稳态和乙烯生物合成中发挥作用。
Hydrogen sulfide (H2S) is involved in multiple processes during plant growth and development. D-cysteine desulfhydrase (DCD) can produce H2S with D-cysteine as the substrate; however, the potential developmental roles of DCD have not been explored during the tomato lifecycle. In the present study, SlDCD2 showed increasing expression during fruit ripening. Compared with the control fruits, the silencing of SlDCD2 by pTRV2-SlDCD2 accelerated fruit ripening. A SlDCD2 gene-edited mutant was constructed by CRISPR/Cas9 transformation, and the mutant exhibited accelerated fruit ripening, decreased H2S release, higher total cysteine and ethylene contents, enhanced chlorophyll degradation and increased carotenoid accumulation. Additionally, the expression of multiple ripening-related genes, including NYC1, PAO, SGR1, PDS, PSY1, ACO1, ACS2, E4, CEL2, and EXP was enhanced during the dcd2 mutant tomato fruit ripening. Compared with the wild-type fruits, SlDCD2 mutation induced H2O2 and malondialdehyde (MDA) accumulation in fruits, which led to an imbalance in reactive oxygen species (ROS) metabolism. A correlation analysis indicated that H2O2 content was strongly positively correlated with carotenoids content, ethylene content and ripening-related gene expression and negatively correlated with the chlorophyll content. Additionally, the dcd2 mutant showed earlier leaf senescence, which may be due to disturbed ROS homeostasis. In short, our findings show that SlDCD2 is involved in H2S generation and that the reduction in endogenous H2S production in the dcd2 mutant causes accelerated fruit ripening and premature leaf senescence. Additionally, decreased H2S in the dcd2 mutant causes excessive H2O2 accumulation and increased ethylene release, suggesting a role of H2S and SlDCD2 in modulating ROS homeostasis and ethylene biosynthesis.
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影响因子：
5.6
作者：
Hu K;Peng X;Yao G;Zhou Z;Yang F;Li W;Zhao Y;Li Y;Han Z;Chen X;Zhang H
通讯作者：
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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