Comparative Transcriptome Analysis Identified Candidate Genes Related to Bailinggu Mushroom Formation and Genetic Markers for Genetic Analyses and Breeding.
Comparative Transcriptome Analysis Identified Candidate Genes Related to Bailinggu Mushroom Formation and Genetic Markers for Genetic Analyses and Breeding.
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比较转录组分析鉴定了与白灵菇形成相关的候选基因以及用于遗传分析和育种的遗传标记
DOI：
10.1038/s41598-017-08049-z
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发表时间：
2017-08-24
影响因子：
4.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Fu Y;Dai Y;Yang C;Wei P;Song B;Yang Y;Sun L;Zhang ZW;Li Y
白灵菇（Pleurotuoliensis）是一种主要的商业栽培蘑菇，广泛用于营养，医药和工业应用。然而，蘑菇的遗传结构及其形成的分子机制在很大程度上是未知的。本研究通过对白灵菇菌丝体、原基和子实体发育过程的比较转录组学分析，鉴定子实体发育的调控基因，并开发评价育种材料遗传价值的EST-SSR标记。利用GO和KEGG数据库对形态建成、初级碳水化合物代谢、冷刺激和蓝光反应等过程中的阶段特异性和差异表达的单基因（DEG）进行了鉴定。这些单基因可能有助于白灵菇适应影响结实的遗传和环境因素。基因表达的变化最明显的发生在植物到生殖的过渡，这表明是最活跃的和关键的白灵谷的发展。在此基础上，我们开发了26个多态性和信息丰富的EST-SSR标记，对82个白灵菇育种材料进行了遗传多样性分析。这些EST-SSRs在近缘物种P. erynamivar.阿魏和变种艾琳。遗传群体结构分析表明，中国白灵菇与这两个品种的遗传渗入程度较低，可能是独立进化的。这些发现为白灵菇的育种提供了新的基因、SSR标记和种质资源。
Bailinggu (Pleurotus tuoliensis) is a major, commercially cultivated mushroom and widely used for nutritional, medicinal, and industrial applications. Yet, the mushroom’s genetic architecture and the molecular mechanisms underlying its formation are largely unknown. Here we performed comparative transcriptomic analysis during Bailinggu’s mycelia, primordia, and fruiting body stages to identify genes regulating fruiting body development and develop EST-SSR markers assessing the genetic value of breeding materials. The stage-specific and differentially expressed unigenes (DEGs) involved in morphogenesis, primary carbohydrate metabolism, cold stimulation and blue-light response were identified using GO and KEGG databases. These unigenes might help Bailinggu adapt to genetic and environmental factors that influence fructification. The most pronounced change in gene expression occurred during the vegetative-to-reproductive transition, suggesting that is most active and key for Bailinggu development. We then developed 26 polymorphic and informative EST-SSR markers to assess the genetic diversity in 82 strains of Bailinggu breeding materials. These EST-SSRs exhibited high transferability in closely related species P. eryngii var. ferulae and var. eryngii. Genetic population structure analysis indicated that China’s Bailinggu has low introgression with these two varieties and likely evolved independently. These findings provide new genes, SSR markers, and germplasm to enhance the breeding of commercially cultivated Bailinggu.
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影响因子：
4.6
作者：
Chen W;Liu YX;Jiang GF
通讯作者：
Jiang GF
杏鲍菇托里菌亚种菌丝体对冷刺激反应的从头测序和转录组分析
DOI：
10.3390/molecules21050560
发表时间：
2016-05-17
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Fu YP;Liang Y;Dai YT;Yang CT;Duan MZ;Zhang Z;Hu SN;Zhang ZW;Li Y
通讯作者：
Li Y
影响因子：
1.9
作者：
Alam N;Shim MJ;Lee MW;Shin PG;Yoo YB;Lee TS
通讯作者：
Lee TS
影响因子：
9.4
作者：
Ceccaroli, P.;Buffalini, M.;Stocchi, V.
通讯作者：
Stocchi, V.
影响因子：
2.8
作者：
He, Xiao-Lan;Wu, Bo;Gan, Bing-Cheng
通讯作者：
Gan, Bing-Cheng