Fos-activation of FoxP2 and Lmx1b neurons in the parabrachial nucleus evoked by hypotension and hypertension in conscious rats.
Fos-activation of FoxP2 and Lmx1b neurons in the parabrachial nucleus evoked by hypotension and hypertension in conscious rats.
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DOI：
10.1016/j.neuroscience.2012.05.049
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发表时间：
2012-08-30
期刊：
影响因子：
3.3
通讯作者：
Loewy, A. D.
中科院分区：
文献类型：
--
作者：
Miller, R. L.;Knuepfer, M. M.;Wang, M. H.;Denny, G. O.;Gray, P. A.;Loewy, A. D.
臂旁核（PB）是一个脑干细胞群，它接收来自孤束核的关于内脏器官生理状态的强输入，并在整个前脑发送传出投射。由于PB的神经解剖组织尚不清楚，我们的第一步是使用针对两种神经谱系转录因子的特异性抗体：叉头盒蛋白2（FoxP 2）和LIM同源域转录因子1 β（Lmx 1b）来定义成年大鼠的PB。这使我们能够构建一个cytoarchitectonic PB地图的基础上，组成性表达这两个转录因子的神经元的分布。第二，原位杂交结合免疫组织化学的方法表明，谷氨酸囊泡转运蛋白V17 a6（Slc 17 a6）的mRNA存在于大多数Lmx 1b+和FoxP 2+臂旁神经元，表明这些神经元使用谷氨酸作为递质。第三，将清醒大鼠保持在肿胀或高血压状态两小时，然后通过标准c-Fos方法制备其脑干，该方法是神经元活性的测量。低血压和高血压均导致PB外外侧-外亚部（PBel-outer）中Lmx 1b+神经元的c-Fos激活。低血压，而不是高血压，引起的FoxP 2+神经元的中央外侧PB（PBcl）亚核的c-Fos活性。在Köliker-Ehrman核以及外侧新月PB和PBcl的喙部含有共表达FoxP 2+和Lmx 1b+的神经元，但这些神经元在血压变化后均未被激活。在血压变化后，前蓝斑和PBel内的盐敏感FoxP 2神经元不被c-Fos激活。总之，本研究表明PBel-外亚核和PBcl亚核起源于两种不同的神经祖细胞，含有多巴胺能神经元，并受到血压变化的影响，PBel-外亚核对低血压和高血压都有反应，而PBcl信号仅发生过度变化。
The parabrachial nucleus (PB) is a brainstem cell group that receives a strong input from the nucleus tractus solitarius regarding the physiological status of the internal organs and sends efferent projections throughout the forebrain. Since the neuroanatomical organization of the PB remains unclear, our first step was to use specific antibodies against two neural lineage transcription factors: Forkhead box protein2 (FoxP2) and LIM homeodomain transcription factor 1 beta (Lmx1b) to define the PB in adult rats. This allowed us to construct a cytoarchitectonic PB map based on the distribution of neurons that constitutively express these two transcription factors. Second, the in situ hybridization method combined with immunohistochemistry demonstrated that mRNA for glutamate vesicular transporter Vglut2 (Slc17a6) was present in most of the Lmx1b+ and FoxP2+ parabrachial neurons, indicating these neurons use glutamate as a transmitter. Third, conscious rats were maintained in a hypotensive or hypertensive state for two hours, and then, their brainstems were prepared by the standard c-Fos method which is a measure of neuronal activity. Both hypotension and hypertension resulted in c-Fos activation of Lmx1b+ neurons in the external lateral-outer subdivision of the PB (PBel-outer). Hypotension, but not hypertension, caused c-Fos activity in the FoxP2+ neurons of the central lateral PB (PBcl) subnucleus. The Kölliker-Fuse nucleus as well as the lateral crescent PB and rostralmost part of the PBcl contain neurons that co-express FoxP2+ and Lmx1b+, but none of these were activated after blood pressure changes. Salt-sensitive FoxP2 neurons in the pre-locus coeruleus and PBel-inner were not c-Fos activated following blood pressure changes. In summary, the present study shows that the PBel-outer and PBcl subnuclei originate from two different neural progenitors, contain glutamatergic neurons, and are affected by blood pressure changes, with the PBel-outer reacting to both hypo- and hypertension, and the PBcl signaling only hypotensive changes.
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