Consumption of pasteurized human lysozyme transgenic goats' milk alters serum metabolite profile in young pigs.
Consumption of pasteurized human lysozyme transgenic goats' milk alters serum metabolite profile in young pigs.
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DOI：
10.1007/s11248-009-9334-4
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发表时间：
2010-08
影响因子：
3
通讯作者：
Murray JD
中科院分区：
文献类型：
--
作者：
Brundige DR;Maga EA;Klasing KC;Murray JD
营养、胃肠道细菌组成和总体健康状况都会影响生物体的代谢特征。我们之前已经证明，喂养表达人溶菌酶（hLZ）的巴氏杀菌转基因羊奶可以积极影响仔猪的肠道形态和调节肠道微生物群组成。本研究的目的是通过分析血清代谢物，进一步研究食用含hlz牛奶对仔猪的影响。将猪分为两组，分别饲喂固体食物和对照（非转基因）羊奶或转基因hlz羊奶，为期6周。在饲喂期结束时采集血清样本，并进行总体代谢物分析。共获得225种代谢物（160种已知，65种未知）的半定量数据。18种已知代谢物和4种未知代谢物的水平在两组之间存在显著差异，其中13种已知代谢物的变化方向几乎完全符合健康状况的改善，特别是在胃肠道健康和免疫反应方面，其他5种代谢物的影响是中性或未知的。这些结果进一步支持了我们的假设，即食用含hlz的牛奶对健康有益。
Nutrition, bacterial composition of the gastrointestinal tract, and general health status can all influence the metabolic profile of an organism. We previously demonstrated that feeding pasteurized transgenic goats’ milk expressing human lysozyme (hLZ) can positively impact intestinal morphology and modulate intestinal microbiota composition in young pigs. The objective of this study was to further examine the effect of consuming hLZ-containing milk on young pigs by profiling serum metabolites. Pigs were placed into two groups and fed a diet of solid food and either control (non-transgenic) goats’ milk or milk from hLZ-transgenic goats for 6 weeks. Serum samples were collected at the end of the feeding period and global metabolite profiling was performed. For a total of 225 metabolites (160 known, 65 unknown) semi-quantitative data was obtained. Levels of 18 known and 4 unknown metabolites differed significantly between the two groups with the direction of change in 13 of the 18 known metabolites being almost entirely congruent with improved health status, particularly in terms of the gastrointestinal tract health and immune response, with the effects of the other five being neutral or unknown. These results further support our hypothesis that consumption of hLZ-containing milk is beneficial to health.
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影响因子：
7.7
作者：
CLEMENTS, RS;REYNERTSON, R
通讯作者：
REYNERTSON, R
影响因子：
3.3
作者：
DUNSHEA, FR;BAUMAN, DE;BELL, AW
通讯作者：
BELL, AW
影响因子：
7.1
作者：
CLEMENTS, RS;DARNELL, B
通讯作者：
DARNELL, B
DOI：
10.1007/bf03190428
发表时间：
2002-04-01
影响因子：
1.9
作者：
Banoglu, E;King, RS
通讯作者：
King, RS
影响因子：
3.6
作者：
Bertram, Hanne Christine;Hoppe, Camilla;Michaelsen, Kim F.
通讯作者：
Michaelsen, Kim F.