Electrospun core-shell nanofibers with encapsulated enamel matrix derivative for guided periodontal tissue regeneration.
Electrospun core-shell nanofibers with encapsulated enamel matrix derivative for guided periodontal tissue regeneration.
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DOI：
10.4012/dmj.2020-412
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发表时间：
2021-09-30
影响因子：
2.5
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Lam LRW;Schilling K;Romas S;Misra R;Zhou Z;Caton JG;Zhang X
评价了包封有Emdogain®（EMD）的复合电纺核-壳生物膜的成骨作用。以聚己内酯为壳层，聚乙二醇为芯层，采用同轴静电纺丝法制备了聚己内酯/聚乙二醇共混膜。采用茜素红S染色和qRT-PCR检测膜对牙周膜干细胞（PDLSCs）成骨分化的影响。膜的表征表明核-壳形态，平均直径约为1µm。在22天的时间内从核-壳纳米纤维释放异硫氰酸荧光素缀合的牛血清白蛋白（FITC-BSA）的检查表明，与固体纳米纤维相比，包封的蛋白质的释放曲线得到改善。当在含有EMD的核-壳纳米纤维上培养时，PDLSC表现出显着改善的成骨分化，茜素红S染色增加，成骨基因表达增强，即OCN，RUNX 2，ALP和OPN。核壳结构的牙周膜可同时发挥机械屏障和药物控释功能，从而改善牙周再生治疗的效果。
The osteogenic effect of a composite electrospun core-shell nanofiber membrane encapsulated with Emdogain® (EMD) was evaluated. The membrane was developed through coaxial electrospinning using polycaprolactone as the shell and polyethylene glycol as the core. The effects of the membrane on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) were examined using Alizarin Red S staining and qRT-PCR. Characterization of the nanofiber membrane demonstrated core-shell morphology with a mean diameter of ~1µm. Examination of the release of fluorescein isothiocyanate-conjugated bovine serum albumin (FITC-BSA) from core-shell nanofibers over a 22-day period showed improved release profile of encapsulated proteins as compared to solid nanofibers. When cultured on EMD-containing core-shell nanofibers, PDLSCs showed significantly improved osteogenic differentiation with increased Alizarin Red S staining and enhanced osteogenic gene expression, namely OCN, RUNX2, ALP, and OPN. Core-shell nanofiber membranes may improve outcomes in periodontal regenerative therapy through simultaneous mechanical barrier and controlled drug delivery function.
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DOI：
10.1016/j.nano.2019.102090
发表时间：
2020-01-01
影响因子：
5.4
作者：
Baek, Jihye;Lee, Emily;D'Lima, Darryl D.
通讯作者：
D'Lima, Darryl D.
影响因子：
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影响因子：
4.3
作者：
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通讯作者：
Genco RJ
影响因子：
3.5
作者：
Nagatomo, K;Komaki, M;Ishikawa, I
通讯作者：
Ishikawa, I
影响因子：
5
作者：
Hu, Jue;Tian, Lingling;Ramakrishna, Seeram
通讯作者：
Ramakrishna, Seeram