Staphylococcus aureus resistance on titanium coated with multivalent PEGylated-peptides.
Staphylococcus aureus resistance on titanium coated with multivalent PEGylated-peptides.
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DOI：
10.1016/j.biomaterials.2010.08.031
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发表时间：
2010-12
期刊：
影响因子：
14
通讯作者：
Grinstaff, Mark W.
中科院分区：
文献类型：
--
作者：
Khoo, Xiaojuan;O'Toole, George A.;Nair, Shrikumar A.;Snyder, Brian D.;Kenan, Daniel J.;Grinstaff, Mark W.
细菌感染会对植入装置的功效、寿命和安全性产生不利影响，并且是骨科植入物失败的第二个最常见原因。我们之前已经展示了在钛上组装聚乙二醇化的钛结合肽（TBP）以获得可以有效抵抗蛋白质吸附和金黄色葡萄球菌（S. aureus）粘附的抗菌表面涂层。在本研究中，我们研究了多个 TBP 重复序列对体外涂层性能的影响。合成单价、二价和四价肽并评估结合亲和力和血清稳定性。制备聚乙二醇化类似物并评估其对金黄色葡萄球菌附着和生物膜形成的影响。涂层性能随着 TBP 重复次数的增加而提高，四价涂层 TBP4-PEG 在所有测定中均显示出最佳性能。特别是，TBP4-PEG 形成抗血清表面涂层，能够防止金黄色葡萄球菌定植和随后的生物膜形成。这些结果进一步支持多价在开发改进的表面涂层中发挥的作用，该表面涂层具有增强的稳定性和体内临床使用的功效。
Bacterial infections can have adverse effects on the efficacy, lifetime and safety of an implanted device and are the second most commonly attributed cause of orthopedic implant failure. We have previously shown the assembly of PEGylated titanium-binding peptides (TBPs) on Ti to obtain a bacteriophobic surface coating that can effectively resist protein adsorption and Staphylococcus aureus (S. aureus) adhesion. In the present study, we examine the effect of multiple TBP repeats on coating performance in vitro. Mono, di, and tetravalent peptides were synthesized and assessed for binding affinity and serum stability. PEGylated analogs were prepared and evaluated for their effect on S. aureus attachment and biofilm formation. Coating performance improved with the number of TBP repeats, with the tetravalent coating, TBP4–PEG, showing the best performance in all assays. In particular, TBP4–PEG forms a serum-resistant surface coating capable of preventing S. aureus colonization and subsequent biofilm formation. These results further support the role that multivalency can play in the development of improved surface coatings with enhanced stabilities and efficacy for in vivo clinical use.
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影响因子：
--
作者：
Dwyer, MA;Lu, WY;Kossiakoff, A
通讯作者：
Kossiakoff, A
影响因子：
5.2
作者：
Hart, Emily;Azzopardi, Kristy;Robins-Browne, Roy
通讯作者：
Robins-Browne, Roy
影响因子：
--
作者：
Kenan, Daniel J.;Walsh, Elisabeth B.;Grinstaff, Mark W.
通讯作者：
Grinstaff, Mark W.
影响因子：
29.4
作者：
Meyers, Steven R.;Hamilton, Paul T.;Grinstaff, Mark W.
通讯作者：
Grinstaff, Mark W.
影响因子：
5.1
作者：
Arciola, Carla Renata;Campoccia, Davide;Montanaro, Lucio
通讯作者：
Montanaro, Lucio