Intramolecular Annulation of Gossypol by Laccase to Produce Safe Cottonseed Protein.
Intramolecular Annulation of Gossypol by Laccase to Produce Safe Cottonseed Protein.
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漆酶对棉酚进行分子内环化生产安全的棉籽蛋白
DOI：
10.3389/fchem.2020.583176
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发表时间：
2020
影响因子：
5.5
通讯作者：
Li P
中科院分区：
文献类型：
--
作者：
Wang L;Chen M;Luo X;Fan Y;Zheng Z;He Z;Yin R;Meng T;Xu S;Pan Y;Su J;Du J;Zhang L;Tian X;Tian Y;Chen D;Ge H;Zhang N;Li P
酚棉酚的存在严重限制了棉籽粕和棉籽油在食品和动物饲料工业中的利用。棉酚的高效生物降解手段及其降解产物的细胞毒性的理解仍然超出目前的知识，是普遍的兴趣。本研究首次证明了漆酶可以催化棉酚中的醛和羟基与邻-半醌自由基的分子内环化反应，并产生·OH自由基。进一步发现，醛基的氧化显著降低生殖毒性和肝毒性。这些结果表明棉酚的一种新的解毒途径，并揭示了自由基物种在环化中发挥的关键作用。这一发现有助于开发安全、方便、低成本的棉花蛋白和油脂资源脱毒工业化方法。
The presence of the phenol gossypol has severely limited the utilization of cottonseed meal and oil in the food and animal feed industries. Highly efficient means of biodegradation of gossypol and an understanding of the cytotoxicity of its degradation products remain outside current knowledge and are of universal interest. In this work, we showed for the first time that laccase can catalyze the intramolecular annulation of the aldehyde and hydroxyl groups of gossypol for the o-semiquinone radical and originate the released ·OH radical. It was further found that the oxidation of aldehyde groups significantly decreases reproductive toxicity and hepatotoxicity. These results indicate a novel detoxification pathway for gossypol and reveal the crucial role played by radical species in cyclization. This discovery could facilitate the development of safe, convenient, and low-cost industrial methods for the detoxification of cotton protein and oil resources.
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影响因子：
4.3
作者：
El-Sharaky, A. S.;Wahby, M. M.;El-Shahawy, I. N.
通讯作者：
El-Shahawy, I. N.
影响因子：
15
作者：
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通讯作者：
Soto-Blanco, Benito
影响因子：
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作者：
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通讯作者：
Rathore, Keerti S.