Novel use of cranial epidural space in rabbits as an animal model to investigate bone volume augmentation potential of different bone graft substitutes.
Novel use of cranial epidural space in rabbits as an animal model to investigate bone volume augmentation potential of different bone graft substitutes.
复制标题
DOI：
10.1186/s13005-016-0131-z
复制
发表时间：
2016-12-01
影响因子：
3
通讯作者：
Manzanares-Céspedes MC
中科院分区：
文献类型：
--
作者：
Valdivia-Gandur I;Engelke W;Beltrán V;Borie E;Fuentes R;Manzanares-Céspedes MC
骨增量的成功在很大程度上取决于周围组织的生物力学和生物学条件。因此，需要一种动物模型来提供具有相似机械压力的解剖部位，以比较其对不同生物材料骨再生的影响。本报告描述了在脑脊液恒定压力下，在硬膜外腔、硬脑膜和颅骨之间产生的滑囊中与生物材料相关的新骨形成。五只成年加州兔被用于试验。在每只动物中，在颅骨前部、额间缝两侧下方的硬膜外腔中形成两个滑囊。硬脑膜和颅骨之间的空间填充有原位硬化双相磷酸钙，其中含有羟基磷灰石和β-磷酸三钙（BCP）、原位硬化相纯β-磷酸三钙（β-TCP）或不含任何生物材料（假手术）。 90 天后，处死动物，提取缺陷部位并进行处理，通过光学和背散射电子显微镜进行组织形态分析。在所有实验点应用 BCP (n = 3) 和 β-TCP 生物材料 (n = 3) 可以保存所创建的颅骨硬膜外空间 (n = 10)。与用 β-TCP 增强的部位相比，用 BCP 增强的部位显示出较少的新骨形成，但具有更好的体积保留趋势。然而，BCP 部位的骨骼似乎更成熟，这些部位的板层骨百分比较高，表明该部位的骨骼较成熟。相比之下，假手术部位无法保留所创建的空间，并且新骨形成很少（n = 4）。在硬膜外腔双侧创建的实验滑囊可以客观地比较骨形成与生物材料的关系，以在脑脊液压力的永久生理力下进行骨再生。
The success of bone augmentation to a major degree depends on the biomechanics and biological conditions of the surrounding tissues. Therefore, an animal model is needed providing anatomical sites with similar mechanical pressures for comparing its influence on different biomaterials for bone regeneration. The present report describes the new bone formation associated to biomaterial in a bursa created in the epidural space, between dura mater and cranial calvaria, under the constant pressure of cerebrospinal fluid. Five adult California rabbits were used for the trial. In each animal, two bursae were created in the epidural spaces, in the anterior part of the skull, below both sides of the interfrontal suture. The spaces between dura mater and cranial calvaria were filled with in-situ hardening biphasic calcium phosphate containing hydroxyapatite and beta tricalcium-phosphate (BCP), in-situ hardening phase-pure beta-tricalcium phosphate (β-TCP) or without any biomaterials (sham). After 90 days, the animals were sacrificed, and the defect sites were extracted and processed for histomorphometric analysis by optical and backscattered electron microscopy. The cranial epidural spaces created (n = 10) could be preserved by the application both BCP (n = 3) and β-TCP biomaterials (n = 3) in all experimental sites. The sites augmented with BCP showed less new bone formation but a trend to better volume preservation than the sites augmented with β-TCP. However, the bone in the BCP sites seemed to be more mature as indicated by the higher percentage of lamellar bone in the sites. In contrast, the created space could not be preserved, and new bone formation was scarce in the sham-operated sites (n = 4). The experimental bursae created bilaterally in the epidural space allows comparing objectively bone formation in relation to biomaterials for bone regeneration under permanent physiological forces from cerebrospinal fluid pressure.
登录
查看更多内容
影响因子：
--
作者：
Inagaki Y;Uematsu K;Akahane M;Morita Y;Ogawa M;Ueha T;Shimizu T;Kura T;Kawate K;Tanaka Y
通讯作者：
Tanaka Y
影响因子：
2.2
作者：
Borie, Eduardo;Fuentes, Ramon;Engelke, Wilfried
通讯作者：
Engelke, Wilfried
影响因子：
6.4
作者：
Klijn, Reinoud J.;Meijer, Gert J.;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
3.1
作者：
Metzler, Philipp;von Wilmowsky, Cornelius;Schlegel, Karl Andreas
通讯作者：
Schlegel, Karl Andreas
影响因子：
4.3
作者：
Favero, Vittorio;Lang, Niklaus P.;Botticelli, Daniele
通讯作者：
Botticelli, Daniele