Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits.
Osteogenic matrix cell sheet transplantation enhances early tendon graft to bone tunnel healing in rabbits.
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DOI：
10.1155/2013/842192
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发表时间：
2013
影响因子：
--
通讯作者：
Tanaka Y
中科院分区：
文献类型：
--
作者：
Inagaki Y;Uematsu K;Akahane M;Morita Y;Ogawa M;Ueha T;Shimizu T;Kura T;Kawate K;Tanaka Y
本研究的目的是确定成骨基质细胞片（OMCS）是否可以诱导移植肌腱周围的骨形成，从而促进早期肌腱骨隧道愈合的雄性日本白色兔。首先，评价兔OMCS的成骨潜力。然后，将OMCS移植到移植肌腱与胫骨处创建的骨隧道之间的界面中。3周后进行组织学评估和生物力学拉伸试验。兔OMCS与β-磷酸三钙片皮下移植后，显示出高碱性磷酸酶（ALP）活性，ALP阳性染色，并具有成骨潜能。OMCS移植组肌腱周围可见新骨壁形成和I型胶原染色阳性，而OMCS移植组肌腱周围骨壁较薄或较少见。显微计算机断层扫描图像显示OMCS移植组的骨体积显著更高。使用OMCS的拔出强度（0.74 ± 0.23 N/mm 2）显著高于不使用OMCS的拔出强度（0.58 ± 0.15 N/mm 2）。这些结果表明，OMCS促进早期肌腱-骨隧道愈合。这种方法可以应用于韧带重建手术后需要早期肌腱到骨隧道愈合的病例。
The objective of this study was to determine whether osteogenic matrix cell sheets (OMCS) could induce bone formation around grafted tendons, thereby enhancing early stage tendon to bone tunnel healing in skeletally mature male Japanese white rabbits. First, the osteogenic potential of rabbit OMCS was evaluated. Then, the OMCS were transplanted into the interface between the grafted tendon and the bone tunnel created at the tibia. Histological assessments and biomechanical tensile testing were performed after 3 weeks. The rabbit OMCS showed high alkaline phosphatase (ALP) activity, positive staining of ALP, and osteogenic potential when transplanted subcutaneously with beta tricalcium phosphate disks. Newly formed bony walls and positive collagen type I staining were seen around the grafted tendon with OMCS transplantation, whereas such bony walls were thinner or less frequent without OMCS transplantation. Micro-computed tomography images showed significantly higher bone volume in the OMCS transplantation group. The pullout strength was significantly higher with OMCS (0.74 ± 0.23 N/mm2) than without OMCS (0.58 ± 0.15 N/mm2). These results show that OMCS enhance early tendon to bone tunnel healing. This method can be applied to cases requiring early tendon to bone tunnel healing after ligament reconstruction surgery.
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影响因子：
3.7
作者：
Hanifi A;McCarthy H;Roberts S;Pleshko N
通讯作者：
Pleshko N
影响因子：
2.8
作者：
Yeh, Wen-Ling;Lin, Song-Shu;Ueng, Steve W. N.
通讯作者：
Ueng, Steve W. N.
影响因子：
11.1
作者：
Elloumi-Hannachi, I.;Yamato, M.;Okano, T.
通讯作者：
Okano, T.
DOI：
10.1016/j.arthro.2004.08.010
发表时间：
2004-12-01
影响因子：
4.7
作者：
Yasuda, K;Kondo, E;Minami, A
通讯作者：
Minami, A
影响因子：
3.3
作者：
Akahane, Manabu;Nakamura, Akifumi;Takakura, Yoshinori
通讯作者：
Takakura, Yoshinori