Bright Chromenylium Polymethine Dyes Enable Fast, Four-Color In Vivo Imaging with Shortwave Infrared Detection.
Bright Chromenylium Polymethine Dyes Enable Fast, Four-Color In Vivo Imaging with Shortwave Infrared Detection.
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DOI：
10.1021/jacs.0c11599
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发表时间：
2021-05-12
影响因子：
15
通讯作者：
Sletten EM
中科院分区：
文献类型：
--
作者：
Cosco ED;Arús BA;Spearman AL;Atallah TL;Lim I;Leland OS;Caram JR;Bischof TS;Bruns OT;Sletten EM
在电磁波谱的短波红外（SWIR，1000-2000 nm）区域内的光学成像已经能够在小鼠中非侵入性地进行高分辨率和高对比度成像。聚甲川染料具有窄的吸收光谱和高的吸收系数，是快速和多路复用SWIR成像的最佳探针。在这里，我们扩大后，在短波红外成像的多路复用能力，获得更明亮的聚甲炔染料与不同的激发波长间隔在整个近红外（700-1000 nm）区域。在黄酰亚胺多甲川染料支架的基础上，我们探索了在2位具有官能团取代的衍生物，认为是色烯酰亚胺多甲川染料。报道的染料具有降低的非辐射率和增强的发射特性，能够以300 fps的单色和100 fps的三种颜色在小鼠中进行非侵入性成像。与含有红移的久洛尼定黄环和吲哚菁绿色的聚甲炔染料组合，具有良好分离的激发波长的不同通道提供四种颜色的非侵入性视频速率体内成像。
Optical imaging within the shortwave infrared (SWIR, 1000–2000 nm) region of the electromagnetic spectrum has enabled high-resolution and high-contrast imaging in mice, non-invasively. Polymethine dyes, with their narrow absorption spectra and high absorption coefficients, are optimal probes for fast and multiplexed SWIR imaging. Here, we expand upon the multiplexing capabilities in SWIR imaging by obtaining brighter polymethine dyes with varied excitation wavelengths spaced throughout the near-infrared (700–1000 nm) region. Building on the flavylium polymethine dye scaffold, we explored derivatives with functional group substitution at the 2-position, deemed chromenylium polymethine dyes. The reported dyes have reduced nonradiative rates and enhanced emissive properties, enabling non-invasive imaging in mice in a single color at 300 fps and in three colors at 100 fps. Combined with polymethine dyes containing a red-shifted julolidine flavylium heterocycle and indocyanine green, distinct channels with well-separated excitation wavelengths provide non-invasive video-rate in vivo imaging in four colors.
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影响因子：
16.6
作者：
Lei, Zuhai;Li, Xinran;Yang, Youjun
通讯作者：
Yang, Youjun
影响因子：
41.2
作者：
Lu GJ;Farhadi A;Szablowski JO;Lee-Gosselin A;Barnes SR;Lakshmanan A;Bourdeau RW;Shapiro MG
通讯作者：
Shapiro MG
影响因子：
28.1
作者：
Bruns, Oliver T.;Bischof, Thomas S.;Bawendi, Moungi G.
通讯作者：
Bawendi, Moungi G.
与激发激光器相匹配的短波红外多胸荧光团可实时实时非侵入性，多色在体内成像。
影响因子：
21.8
作者：
Cosco ED;Spearman AL;Ramakrishnan S;Lingg JGP;Saccomano M;Pengshung M;Arús BA;Wong KCY;Glasl S;Ntziachristos V;Warmer M;McLaughlin RR;Bruns OT;Sletten EM
通讯作者：
Sletten EM
影响因子：
2.9
作者：
Lavis, Luke D.
通讯作者：
Lavis, Luke D.