Characterisation of tropomyosin and paramyosin as vaccine candidate molecules for the poultry red mite, Dermanyssus gallinae.
Characterisation of tropomyosin and paramyosin as vaccine candidate molecules for the poultry red mite, Dermanyssus gallinae.
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DOI：
10.1186/s13071-016-1831-8
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发表时间：
2016-10-12
影响因子：
3.2
通讯作者：
Nisbet AJ
中科院分区：
文献类型：
--
作者：
Wright HW;Bartley K;Huntley JF;Nisbet AJ
鸡皮刺螨是世界范围内商业蛋鸡群中最重要的经济性吸血体外寄生虫。它在夜间侵扰母鸡，引起刺激，导致不安，啄食，在极端情况下贫血和同类相食增加。由于抗杀螨剂D.因此，需要新的控制策略，疫苗接种可能提供一种可持续的替代杀螨剂。在本研究中，重组形式的D.作为疫苗候选分子，生产、表征和测试了鸡原肌球蛋白（Der g 10）和副肌球蛋白（Der g 11）。地方对鸡副肌球蛋白（Der g 11）的编码序列进行了分析，并对Der g 11和D.鸡原肌球蛋白（Der g 10）。用重组蛋白免疫母鸡，并将产生的抗体喂给D。评估鸡和螨的死亡率。用抗Der g 11和Der g 10抗体对螨组织切片进行探针检测，以确定这些蛋白在D.鸡。 Der g 11基因全长2,622 bp，编码874个氨基酸残基.免疫组织化学染色显示Der g 10和Der g 11分布于D.鸡组织。在系统发育分析中，这些蛋白质都与蜱类的直系同源物聚类，而不是与蜱螨的直系同源物。在母鸡中产生的针对这些蛋白的重组形式的抗体显著增加了D。当使用体外饲喂装置饲喂螨时，Der g 10和Der g 11的鸡死亡率分别降低19%（P < 0.001）和23%（P = 0.009）。本研究表明Der g 10和Der g 11在D.并且针对这些蛋白质的重组形式产生的抗体可用于显著增加D.鸡死亡率在体外饲养试验。当比较使用该体外饲养测定评估为疫苗的所有重组和天然蛋白质的存档数据时，Der g 10和Der g 11排名较高，并且表现优于一些天然蛋白质池。
Dermanyssus gallinae is the most economically important haematophagous ectoparasite in commercial egg laying flocks worldwide. It infests the hens during the night where it causes irritation leading to restlessness, pecking and in extreme cases anaemia and increased cannibalism. Due to an increase in the occurrence of acaricide-resistant D. gallinae populations, new control strategies are required and vaccination may offer a sustainable alternative to acaricides. In this study, recombinant forms of D. gallinae tropomyosin (Der g 10) and paramyosin (Der g 11) were produced, characterised and tested as vaccine candidate molecules. The D. gallinae paramyosin (Der g 11) coding sequence was characterised and recombinant versions of Der g 11 and D. gallinae tropomyosin (Der g 10) were produced. Hens were immunised with the recombinant proteins and the resulting antibodies were fed to D. gallinae and mite mortality evaluated. Sections of mites were probed with anti- Der g 11 and Der g 10 antibodies to identify the tissue distribution of these protein in D. gallinae. The entire coding sequence of Der g 11 was 2,622 bp encoding 874 amino acid residues. Immunohistochemical staining of mite sections revealed that Der g 10 and Der g 11 were located throughout D. gallinae tissues. In phylogenetic analyses of these proteins both clustered with orthologues from tick species rather than with orthologues from astigmatid mites. Antibodies raised in hens against recombinant forms of these proteins significantly increased D. gallinae mortality, by 19 % for Der g 10 (P < 0.001) and by 23 % for Der g 11 (P = 0.009) when fed to the mites using an in vitro feeding device. This study has shown that Der g 10 and Der g 11 were located ubiquitously throughout D. gallinae and that antibodies raised against recombinant versions of these proteins can be used to significantly increase D. gallinae mortality in an in vitro feeding assay. When comparing archived data for all recombinant and native proteins assessed as vaccines using this in vitro feeding assay, Der g 10 and Der g 11 ranked highly and performed better than some of the pools of native proteins.
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影响因子：
14.2
作者：
AKI, T;KODAMA, T;ONO, K
通讯作者：
ONO, K
影响因子：
1.9
作者：
Kilpinen, O;Mullens, BA
通讯作者：
Mullens, BA
影响因子：
2.2
作者：
Nisbet, A. J.;Huntley, J. F.;McDevitt, R.
通讯作者：
McDevitt, R.
影响因子：
4
作者：
Bartley K;Wright HW;Huntley JF;Manson ED;Inglis NF;McLean K;Nath M;Bartley Y;Nisbet AJ
通讯作者：
Nisbet AJ
DOI：
10.1016/s0167-4781(98)00006-2
发表时间：
1998-04-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Asturias, JA;Arilla, MC;Palacios, R
通讯作者：
Palacios, R