Rho-kinase and myosin II affect dynamic neural crest cell behaviors during epithelial to mesenchymal transition in vivo.
Rho-kinase and myosin II affect dynamic neural crest cell behaviors during epithelial to mesenchymal transition in vivo.
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DOI：
10.1016/j.ydbio.2008.09.013
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发表时间：
2008-12-15
影响因子：
2.7
通讯作者：
Halloran, Mary C.
中科院分区：
文献类型：
--
作者：
Berndt, Jason D.;Clay, Matthew R.;Langenberg, Tobias;Halloran, Mary C.
神经嵴细胞（NCCs）的诱导和迁移对颅面结构和周围神经系统的发育至关重要。NCCs发展的一个关键步骤是上皮细胞向间充质细胞的转化（EMT），它们经历了上皮细胞向间充质细胞的转化，以启动迁移。几个转录因子对NCC EMT很重要。然而，对于与EMT相关的调节细胞粘附、细胞骨架和细胞运动变化的效应物，以及体内经历EMT的细胞行为的具体变化，我们所知甚少。我们使用斑马鱼后脑ncc的延时成像来显示ncc在EMT期间经历了一系列刻板的行为。我们发现细胞粘附和膜泡的丧失先于丝状延伸和迁移的开始。肌动蛋白动态成像显示肌动蛋白在水泡和丝状足的定位不同。此外，我们发现肌球蛋白II或rho激酶（ROCK）活性的破坏可抑制NCC起泡并导致NCC EMT减少。这些数据揭示了肌球蛋白II和ROCK在体内NCC EMT中的作用，并提供了EMT期间NCC行为的详细特征，这将为进一步的机制研究奠定基础。
The induction and migration of neural crest cells (NCCs) is essential to the development of craniofacial structures and the peripheral nervous system. A critical step in the development of NCCs is the epithelial to mesenchymal transition (EMT) that they undergo in order to initiate migration. Several transcription factors are important for the NCC EMT. However, less is known about the effectors regulating changes in cell adhesion, the cytoskeleton, and cell motility associated with the EMT or about specific changes in the behavior of cells undergoing EMT in vivo. We used time-lapse imaging of NCCs in the zebrafish hindbrain to show that NCCs undergo a stereotypical series of behaviors during EMT. We find that loss of cell adhesion and membrane blebbing precede filopodial extension and the onset of migration. Live imaging of actin dynamics shows that actin localizes differently in blebs and filopodia. Moreover, we find that disruption of myosin II or Rho-kinase (ROCK) activity inhibits NCC blebbing and causes reduced NCC EMT. These data reveal roles for myosin II and ROCK in NCC EMT in vivo, and provide a detailed characterization of NCC behavior during EMT that will form a basis for further mechanistic studies.
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DOI：
10.1007/11664550_11
发表时间：
2006-01-01
期刊：
CONTROL OF UNCERTAIN SYSTEMS: MODELLING, APPROXIMATION, AND DESIGN
影响因子：
--
作者：
Effler, JC;Iglesias, PA;Robinson, DN
通讯作者：
Robinson, DN
DOI：
10.1002/bdrc.20011
发表时间：
2004-06-01
期刊：
Birth defects research. Part C, Embryo today : reviews
影响因子：
--
作者：
Heeg-Truesdell, Elizabeth;LaBonne, Carole
通讯作者：
LaBonne, Carole
影响因子：
2.7
作者：
BRONNERFRASER, M;WOLF, JJ;MURRAY, BA
通讯作者：
MURRAY, BA
影响因子：
4.6
作者：
Burstyn-Cohen, T;Stanleigh, J;Kalcheim, C
通讯作者：
Kalcheim, C
影响因子：
11.8
作者：
Burstyn-Cohen, T;Kalcheim, C
通讯作者：
Kalcheim, C