Ciliate and bacterial communities associated with White Syndrome and Brown Band Disease in reef-building corals.
Ciliate and bacterial communities associated with White Syndrome and Brown Band Disease in reef-building corals.
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DOI：
10.1111/j.1462-2920.2012.02746.x
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发表时间：
2012-08
影响因子：
5.1
通讯作者：
Bythell J
中科院分区：
文献类型：
--
作者：
Sweet M;Bythell J
白色综合征（WS）和褐带病（BrB）是珊瑚礁珊瑚死亡的重要原因，其病因尚未明确确定。在这里，我们使用不依赖培养的分子技术（DGGE和克隆文库）来表征这些疾病的纤毛虫和细菌群落。细菌（16S rRNA基因）和纤毛虫（18S rRNA基因）群落在两种疾病之间高度相似。在两种疾病中均观察到4种细菌和9种纤毛虫核型，但在未患病标本中不存在。只有一种细菌Arcobacter sp. （JF831360）在16S rRNA基因的相对丰度上显著增加，并且在所有患病样本中都有一致的表达。在检测到的11种纤毛虫形态中，有4种含有珊瑚藻类共生体，表明它们摄入了珊瑚组织。在WS和BrB中，两种纤毛形态在所有病变样本中都一致。观察到Morph1 （JN626268）在病变边界的珊瑚组织中钻洞并在其下方钻洞。先前在BrB中发现的Morph2 （JN626269）在发病机制中似乎起次要的、侵袭性较小的作用，但在BrB中具有较高的种群密度，产生可见的棕色带。这些疾病的细菌和纤毛虫群落组成的高度相似性表明它们实际上是相同的综合征。
White Syndrome (WS) and Brown Band Disease (BrB) are important causes of reef coral mortality for which causal agents have not been definitively identified. Here we use culture-independent molecular techniques (DGGE and clone libraries) to characterize ciliate and bacterial communities in these diseases. Bacterial (16S rRNA gene) and ciliate (18S rRNA gene) communities were highly similar between the two diseases. Four bacterial and nine ciliate ribotypes were observed in both diseases, but absent in non-diseased specimens. Only one of the bacteria, Arcobacter sp. (JF831360) increased substantially in relative 16S rRNA gene abundance and was consistently represented in all diseased samples. Four of the eleven ciliate morphotypes detected contained coral algal symbionts, indicative of the ingestion of coral tissues. In both WS and BrB, there were two ciliate morphotypes consistently represented in all disease lesion samples. Morph1 (JN626268) was observed to burrow into and underneath the coral tissues at the lesion boundary. Morph2 (JN626269), previously identified in BrB, appears to play a secondary, less invasive role in pathogenesis, but has a higher population density in BrB, giving rise to the visible brown band. The strong similarity in bacterial and ciliate community composition of these diseases suggests that they are actually the same syndrome.
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影响因子：
2.6
作者：
Efrony, Rotem;Atad, Ilil;Rosenberg, Eugene
通讯作者：
Rosenberg, Eugene
DOI：
10.1099/ijs.0.02359-0
发表时间：
2003-07-01
影响因子：
2.8
作者：
Denner, EBM;Smith, GW;Richardson, LL
通讯作者：
Richardson, LL
影响因子：
1.4
作者：
Barash, Y;Sulam, R;Rosenberg, E
通讯作者：
Rosenberg, E
DOI：
10.1099/ijs.0.02402-0
发表时间：
2003-01-01
影响因子：
2.8
作者：
Ben-Haim, Y;Thompson, FL;Rosenberg, E
通讯作者：
Rosenberg, E
影响因子：
4.4
作者：
Frias-Lopez, J;Bonheyo, GT;Fouke, BW
通讯作者：
Fouke, BW