A NIR-remote controlled upconverting nanoparticle: an improved tool for living cell dye-labeling
A NIR-remote controlled upconverting nanoparticle: an improved tool for living cell dye-labeling
复制标题
近红外遥控上转换纳米粒子：一种改进的活细胞染料标记工具
DOI：
10.1088/0957-4484/26/42/425102
复制
发表时间：
2015-10
期刊：
影响因子：
3.5
通讯作者：
Chang, Jin
中科院分区：
文献类型：
--
作者：
Wang, Huiquan;Li, Wei;Tan, Jian;Chang, Jin
在活细胞中，由于选择性渗透和复杂的细胞环境，在染料标记过程中有机染料的摄取效率和荧光衰减可能会受到影响，这可能最终导致较差的荧光成像。本文设计并成功制备了UCNs@mSiO2-（FA和Azo）核壳纳米载体。核-壳纳米载体由两部分组装而成，包括经叶酸（FA）和偶氮苯（Azo）修饰的介孔二氧化硅壳表面和上转换荧光素（UCN）核。介孔二氧化硅壳用于负载有机染料和缀合叶酸，这有助于增强纳米载体的细胞摄取。UCN核心作为换能器将近红外（NIR）光转换为局部UV和可见光，以激活表面上偶氮苯分子的来回摆动运动，而偶氮苯则作为分子叶轮，推动有机染料的释放。负载有机染料的纳米载体比游离有机染料更能保持荧光成像效果的稳定性。实验结果表明，该纳米颗粒的加入显著提高了细胞对罗丹明和4′，6-二脒基-2-苯基吲哚（DAPI）模型染料的摄取效率。染料的释放只能由NIR光曝光触发，并且它们的量高度依赖于NIR光曝光的持续时间，从而实现NIR调节的染料时空释放。我们的工作可能为生物技术和诊断中精确控制基于UCN的活细胞成像以及研究细胞动力学，细胞间相互作用和组织形态发生开辟了一条新途径。
In living cells, due to the selective permeability and complicated cellular environment, the uptake efficiency and fluorescence decay of organic dyes during dye-labeling may be influenced, which may eventually result in poor fluorescent imaging. In this work, a protocol of UCNs@mSiO2-(FA and Azo) core–shell nanocarriers was designed and prepared successfully. The core–shell nanocarriers were assembled from two parts, including a mesoporous silica shell surface modified by folate (FA) and azobenzene (Azo), and an upconverting nanocrystal (UCN) core. The mesoporous silica shell is used for loading organic dyes and conjugating folate which helps to enhance the cellular uptake of nanocarriers. The UCN core works as a transducer to convert near infrared (NIR) light to local UV and visible light to activate a back-and-forth wagging motion of azobenzene molecules on the surface, while the azobenzene acts as a molecular impeller for propelling the release of organic dyes. The nanocarriers of loading organic dyes can maintain the stability of the fluorescent imaging effect better than free organic dyes. The experimental results show that with the help of the nanoparticle, cell uptake efficiency of the model dyes of rhodamine and 4′, 6-diamidino-2-phenylindole (DAPI) was significantly improved. The release of dyes can only be triggered by NIR light exposure and their quantity is highly dependent on the duration of NIR light exposure, thus realizing NIR-regulated dye release spatiotemporally. Our work may open a novel avenue for precisely controlling UCN-based living cell imaging in biotechnology and diagnostics, as well as studying cell dynamics, cell–cell interactions, and tissue morphogenesis.
登录
查看更多内容
DOI：
10.1073/pnas.1114551109
发表时间：
2012-05-29
影响因子：
11.1
作者：
Jayakumar, Muthu Kumara Gnanasammandhan;Idris, Niagara Muhammad;Zhang, Yong
通讯作者：
Zhang, Yong
影响因子：
6.7
作者：
Li, Chunxia;Liu, Jinliang;Zhang, Yong
通讯作者：
Zhang, Yong
光响应脂质体纳米杂化陶瓷体
影响因子：
4.9
作者：
Liang, Xiaolong;Yue, Xiuli;Kikuchi, Jun-ichi
通讯作者：
Kikuchi, Jun-ichi
影响因子：
4.9
作者：
Trewyn, Brian G.;Giri, Supratim;Lin, Victor S. -Y.
通讯作者：
Lin, Victor S. -Y.
影响因子：
3.8
作者：
Hanjie Wang;P. Zhao;Xiaofei Liang;Tao Song;Xiaoqun Gong;R. Niu;Jin Chang
通讯作者：
Hanjie Wang;P. Zhao;Xiaofei Liang;Tao Song;Xiaoqun Gong;R. Niu;Jin Chang