Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.
Solving the jigsaw puzzle of wound-healing potato cultivars: metabolite profiling and antioxidant activity of polar extracts.
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DOI：
10.1021/jf501330h
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发表时间：
2014-08-06
影响因子：
6.1
通讯作者：
Stark RE
中科院分区：
文献类型：
--
作者：
Dastmalchi K;Cai Q;Zhou K;Huang W;Serra O;Stark RE
马铃薯（Solanum tuberosum L.）是世界范围内的主食，但由于外皮的损伤和随后不利的愈合条件，这种作物的种植伴随着大量的浪费。出于经济和营养方面的考虑，这项代谢产物分析研究旨在提高对闭合层和伤口周围组织形成的理解，并指导新方法的开发，以确保皮肤破损后更快，更完全的愈合。在创伤后3天和7天，分别在栓化封闭层形成和新生创伤围壁组织发育期间，分析了4个马铃薯品种（Norkotah Russet、Atlantic、Chipeta和育空地区Gold）的创伤愈合组织的极性代谢物，这些马铃薯品种具有不同的块茎皮肤锈色化模式。使用LC-MS和NMR光谱方法评估极性提取物，包括多变量分析和24种生物标志物中的22种的初步鉴定，这些生物标志物在给定的伤口愈合时间点或发育阶段之间区分品种。可以从NMR和MS衍生的生物标志物中鉴定的代谢物之间的差异突出了每种方法的优势和局限性，也证明了这些方法在组装组织提取物的完整分子图像方面的互补性。这两种方法都表明，在受伤后的愈合过程中，品种间的代谢产物谱的差异减少。生物标志物包括多酚胺、黄酮苷、酚酸和配糖生物碱。由于伤口愈合与氧化应激有关，因此在每个创伤时间点测量来自不同品种的提取物的自由基清除活性，揭示了育空地区金紫苏的显著更高的清除活性，尤其是在创伤7天后。
Potato (Solanum tuberosum L.) is a worldwide food staple, but substantial waste accompanies the cultivation of this crop due to wounding of the outer skin and subsequent unfavorable healing conditions. Motivated by both economic and nutritional considerations, this metabolite profiling study aims to improve understanding of closing layer and wound periderm formation and guide the development of new methods to ensure faster and more complete healing after skin breakage. The polar metabolites of wound-healing tissues from four potato cultivars with differing patterns of tuber skin russeting (Norkotah Russet, Atlantic, Chipeta, and Yukon Gold) were analyzed at three and seven days after wounding, during suberized closing layer formation and nascent wound periderm development, respectively. The polar extracts were assessed using LC-MS and NMR spectroscopic methods, including multivariate analysis and tentative identification of 22 of the 24 biomarkers that discriminate among the cultivars at a given wound-healing time point or between developmental stages. Differences among the metabolites that could be identified from NMR- and MS-derived biomarkers highlight the strengths and limitations of each method, also demonstrating the complementarity of these approaches in terms of assembling a complete molecular picture of the tissue extracts. Both methods revealed that differences among the cultivar metabolite profiles diminish as healing proceeds during the period following wounding. The biomarkers included polyphenolic amines, flavonoid glycosides, phenolic acids and glycoalkaloids. Because wound healing is associated with oxidative stress, the free radical scavenging activities of the extracts from different cultivars were measured at each wounding time point, revealing significantly higher scavenging activity of the Yukon Gold periderm especially after 7 days of wounding.
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影响因子：
3.8
作者：
Gomez-Romero, Maria;Segura-Carretero, Antonio;Fernandez-Gutierrez, Alberto
通讯作者：
Fernandez-Gutierrez, Alberto
影响因子：
4.9
作者：
Matsuda, F;Morino, K;Miyagawa, H
通讯作者：
Miyagawa, H
影响因子：
1.4
作者：
Cornelius, Marli T. F.;Carvalho, Mário G. de;Braz-Filho, Raimundo
通讯作者：
Braz-Filho, Raimundo
DOI：
10.1007/bf02851598
发表时间：
1997-05-01
期刊：
AMERICAN POTATO JOURNAL
影响因子：
--
作者：
Love, SL;Werner, BK;Pavek, JJ
通讯作者：
Pavek, JJ
影响因子：
8.8
作者：
Ieri, Francesca;Innocenti, Marzia;Mulinacci, Nadia
通讯作者：
Mulinacci, Nadia