Friction Force Microscopy of Lubricin and Hyaluronic Acid between Hydrophobic and Hydrophilic Surfaces.
Friction Force Microscopy of Lubricin and Hyaluronic Acid between Hydrophobic and Hydrophilic Surfaces.
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DOI：
10.1039/b907155e
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发表时间：
2009-09-21
期刊：
影响因子：
3.4
通讯作者：
Zauscher S
中科院分区：
文献类型：
--
作者：
Chang DP;Abu-Lail NI;Coles JM;Guilak F;Jay GD;Zauscher S
润滑素和透明质酸 (HA) 是滑液的分子成分，长期以来一直被认为在关节润滑和磨损保护中发挥作用。虽然润滑素已被证明具有边界润滑剂的功能，但关于透明质酸的边界润滑能力存在相互矛盾的证据。在这里，我们使用胶体力显微镜来探索这两种分子在生理缓冲溶液中化学均匀的亲水性（羟基封端）和疏水性（甲基封端）表面之间的微观摩擦行为。两个表面上的行为在生理上都是相关的，因为异质关节软骨表面同时包含亲水和疏水元素。疏水表面之间的摩擦最初很高（μ=1.1，施加正常载荷为 100nN），并且通过添加润滑剂显着降低，而亲水表面之间的摩擦最初很低（μ=0.1）并且通过添加润滑剂略有增加。当润滑素浓度高于 200 µg/ml 时，两个表面上的摩擦行为相似 (μ=0.2)，表明两个表面之间的几乎所有相互作用都是在吸附的润滑素分子之间而不是表面本身之间。相比之下，添加 HA 并没有明显改变模型表面之间的摩擦行为。在润滑素和透明质酸的生理混合物中没有发现对摩擦行为的协同作用。润滑素可以同等地介导亲水性和疏水性表面之间的摩擦响应，在足够浓度下可能完全防止直接的表面与表面接触，而HA提供的边界润滑要少得多。
Lubricin and hyaluronic acid (HA), molecular constituents of synovial fluid, have long been theorized to play a role in joint lubrication and wear protection. While lubricin has been shown to function as a boundary lubricant, conflicting evidence exists as to the boundary lubricating ability of hyaluronic acid. Here, we use colloidal force microscopy to explore the friction behavior of these two molecules on the microscale between chemically uniform hydrophilic (hydroxyl-terminated) and hydrophobic (methyl-terminated) surfaces in physiological buffer solution. Behaviors on both surfaces are physiologically relevant since the heterogeneous articular cartilage surface contains both hydrophilic and hydrophobic elements. Friction between hydrophobic surfaces was initially high (μ=1.1, at 100nN of applied normal load) and was significantly reduced by lubricin addition while friction between hydrophilic surfaces was initially low (μ=0.1) and was slightly increased by lubricin addition. At lubricin concentrations above 200 µg/ml, friction behavior on the two surfaces was similar (μ=0.2) indicating that nearly all interaction between the two surfaces was between adsorbed lubricin molecules rather than between the surfaces themselves. In contrast, addition of HA did not appreciably alter the frictional behavior between the model surfaces. No synergistic effect on friction behavior was seen in a physiological mixture of lubricin and HA. Lubricin can equally mediate the frictional response between both hydrophilic and hydrophobic surfaces, likely fully preventing direct surface-to-surface contact at sufficient concentrations, whereas HA provides considerably less boundary lubrication.
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影响因子：
3.1
作者：
Gleghorn, J. P.;Jones, A. R. C.;Bonassar, L. J.
通讯作者：
Bonassar, L. J.
影响因子：
27.4
作者：
HILLS, BA;BUTLER, BD
通讯作者：
BUTLER, BD
DOI：
10.1243/095441105x69060
发表时间：
2006-01-01
影响因子：
1.8
作者：
Bell, CJ;Ingham, E;Fisher, J
通讯作者：
Fisher, J
影响因子：
27.4
作者：
CHARNLEY, J
通讯作者：
CHARNLEY, J
影响因子：
1.6
作者：
HUTTER, JL;BECHHOEFER, J
通讯作者：
BECHHOEFER, J