Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A.
Purification and characterization of eight class 5 outer membrane protein variants from a clone of Neisseria meningitidis serogroup A.
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DOI：
10.1084/jem.168.2.507
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发表时间：
1988-08-01
期刊：
影响因子：
--
通讯作者：
Moll A
中科院分区：
文献类型：
--
作者：
Achtman M;Neibert M;Crowe BA;Strittmatter W;Kusecek B;Weyse E;Walsh MJ;Slawig B;Morelli G;Moll A
已对公开的用于纯化来自淋病奈瑟氏菌的P.II蛋白的方法进行了修改，以允许纯化来自脑膜炎奈瑟氏菌血清组A细菌的5类蛋白。在冈比亚流行病中检测到的5种5类蛋白电泳变体（a、B、c、d和e）和在西非相同克隆的其他分离株中发现的3种其他变体（f、g和h）已被纯化，产量为6-28 mg。变体c的NH 2-末端氨基酸序列不同于其它5类蛋白质的氨基酸序列，而后者与从血清群C脑膜炎球菌的DNA分析中预测的两种5类蛋白质的序列非常相似，并且与从淋球菌中确定的8种P.II蛋白质的序列非常相似。在c蛋白和其他5类蛋白之间发现了许多其他的调节、化学和血清学差异，因此我们建议将5类蛋白再分为两个亚类。已经分离出区分这两种蛋白质亚类的mAb，并且使用这些抗体的蛋白质印迹使我们能够得出结论，这两种蛋白质亚类在从过去50年的不同流行病和大流行病分离的细菌中发现。
Methods published for the purification of P.II proteins from Neisseria gonorrhoea have been modified to allow the purification of class 5 proteins from Neisseria meningitidis serogroup A bacteria. The five class 5 protein electrophoretic variants detected within an epidemic in the Gambia (a, b, c, d, and e) and three other variants (f, g, and h) found within other isolates of the same clone in West Africa have been purified with yields of 6-28 mg. The NH2-terminal amino acid sequence for variant c differs from those of the other class 5 proteins, whereas the latter are very similar to the sequence predicted for two class 5 proteins from DNA analyses of serogroup C meningococci and determined for 8 P.II proteins from gonococci. Numerous other regulatory, chemical, and serological differences were found between the c protein and the other class 5 proteins such that we recommend that the class 5 proteins be subdivided into two subclasses. mAbs have been isolated that distinguish between these two protein subclasses and Western blotting with these antibodies enabled us to conclude that both protein subclasses were found in bacteria isolated from different epidemics and pandemics of the last 50 yr.
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DOI：
10.1099/00221287-99-2-333
发表时间：
1977-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HECKELS, JE
通讯作者：
HECKELS, JE
影响因子：
3.1
作者：
KAWULA, TH;AHO, EL;CANNON, JG
通讯作者：
CANNON, JG
影响因子：
3.1
作者：
SWANSON, J
通讯作者：
SWANSON, J
影响因子：
15.3
作者：
BLAKE, MS;GOTSCHLICH, EC
通讯作者：
GOTSCHLICH, EC
影响因子：
3.6
作者：
STERN, A;MEYER, TF
通讯作者：
MEYER, TF