Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection.
Cytokine profile, proliferation and phosphorylation of ERK1/2 and Akt in circulating mononuclear cells from individuals during the chronic intestinal phase of Schistosomiasis mansoni infection.
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DOI：
10.1186/1471-2334-12-380
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发表时间：
2012-12-27
影响因子：
3.7
通讯作者：
Cunha-Melo JR
中科院分区：
文献类型：
--
作者：
Oliveira-Prado R;Caldas IR;Teixeira-Carvalho A;Andrade MV;Fares RC;Portugal LM;Gazzinelli A;Corrêa-Oliveira R;Cunha-Melo JR
对曼氏血吸虫的免疫反应的特征是围绕寄生虫卵的肉芽肿反应，这些寄生虫卵被捕获在宿主肝脏中，并且这种反应在疾病的慢性阶段调节免疫反应。在感染的慢性肠道阶段，患者的典型外周血单核细胞（PBMC）反应的特征是对沙门氏菌的反应降低。曼氏菌可溶性虫卵抗原为了更好地了解血吸虫感染，本研究探讨了可溶性虫卵抗原（SEA）和可溶性成虫抗原（SWAP）对血吸虫感染的影响。mansoni对来自生活在同一流行区的感染（XTO）和卵阴性（NI）个体的PBMC中的细胞增殖、细胞因子产生以及ERK 1/2和Akt磷酸化的影响。通过细胞免疫表型染色（细胞术）评价活化状态。细胞增殖实验采用CFSE法。细胞因子检测试验（Th 1和Th 2）通过流式细胞仪微珠检测，Array磷酸化状态通过ELISA检测。对来自非血吸虫病流行区的XTO、NI和BD（献血员）个体进行了比较。与BD组相比，SEA刺激后，XTO组的CD 4 + T淋巴细胞增殖率较低，但NI组无此现象。与BD组相比，在未刺激的培养物中以及在SEA和SWAP刺激后，XTO组中的CD 8 + T细胞增殖率较低。SEA或SWAP刺激后的细胞因子分析显示XTO和NI组中细胞因子模式平衡。ERK 1/2和Akt磷酸化在所有组中仅少量检测到;然而，与NI和BD组相比，在SWAP刺激的XTO组中观察到ERK 1/2磷酸化降低。数据表明，来自感染患者的SEA刺激的CD 4 + T细胞的增殖率低于来自NI组的相同细胞。此外，我们观察到SWAP刺激影响XTO组的ERK 1/2磷酸化。
The immune response to Schistosoma mansoni is characterized by a granulomatous reaction around the parasite eggs that are trapped in the host liver, and this reaction modulates the immune response during the chronic phase of the disease. The typical peripheral blood mononuclear cell (PBMC) response of patients during the chronic intestinal phase of infection is characterized by a decreased response to an S. mansoni soluble egg antigen. To obtain a greater understanding of Schistosoma infections, this study investigated the effects of the soluble egg antigen (SEA) and soluble adult worm antigen (SWAP) of S. mansoni on cellular proliferation, cytokine production, and ERK1/2 and Akt phosphorylation in PBMCs from infected (XTO) and egg-negative (NI) individuals living in the same endemic area. The activation status was evaluated by cell immunophenotypic staining (cytometry). The cell proliferation assay was by CFSE method. Cytokine detection assay (Th1 and Th2) was by Cytometric Bead and Array phosphorylation status was by ELISA. The XTO, NI and BD (blood donor) individuals from an area not endemic for schistosomiasis were compared. The CD4+ T lymphocyte proliferation rate was lower in the XTO group, but not the NI group, after SEA stimulation compared to the BD group. The CD8+ T cell proliferation rate was lower in the XTO group in the unstimulated cultures and after both SEA and SWAP stimulation compared to the BD group. Cytokine analysis after either SEA or SWAP stimulation showed a balanced cytokine pattern in the XTO and NI groups. ERK1/2 and Akt phosphorylation were only marginally detected in all groups; however, a decrease in ERK 1/2 phosphorylation was observed in the SWAP-stimulated XTO group compared to both the NI and BD groups. The data indicate that SEA-stimulated CD4+ T cells from infected patients have a lower proliferation rate than the same cells from the NI group. Furthermore, we observed that SWAP stimulation influences ERK1/2 phosphorylation in the XTO group.
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DOI：
10.1016/0035-9203(92)90441-e
发表时间：
1992-01-01
影响因子：
2.2
作者：
BAHIAOLIVEIRA, LMG;GAZZINELLI, G;CORREAOLIVEIRA, R
通讯作者：
CORREAOLIVEIRA, R
影响因子：
2.2
作者：
Geiger, SM;Massara, CL;Corrêa-Oliveira, R
通讯作者：
Corrêa-Oliveira, R
影响因子：
6.4
作者：
Montenegro, SML;Miranda, P;Wynn, TA
通讯作者：
Wynn, TA
DOI：
10.1590/s0037-86821992000200006
发表时间：
1992-04-01
影响因子：
2
作者：
Gazzinelli, G;Colley, D G
通讯作者：
Colley, D G
DOI：
10.1590/s0100-879x1998000100024
发表时间：
1998-01-01
影响因子：
2.3
作者：
Correa-Oliveira, R;Malaquias, LCC;Gazzinelli, G
通讯作者：
Gazzinelli, G