Investigating the beneficial traits of Trichoderma hamatum GD12 for sustainable agriculture-insights from genomics.
Investigating the beneficial traits of Trichoderma hamatum GD12 for sustainable agriculture-insights from genomics.
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DOI：
10.3389/fpls.2013.00258
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发表时间：
2013
影响因子：
5.6
通讯作者：
Grant M
中科院分区：
文献类型：
--
作者：
Studholme DJ;Harris B;Le Cocq K;Winsbury R;Perera V;Ryder L;Ward JL;Beale MH;Thornton CR;Grant M
钩状木霉菌株GD 12具有促进植物生长、激活对芽前和芽后土壤病原菌的生物防治以及诱导对叶面病原菌的系统抗性的独特性。本研究扩展了先前在莴苣中的工作，以证明GD 12可以赋予其他植物有益的农艺性状，提供了在模式双子叶植物拟南芥中促进植物生长和在模式单子叶植物水稻中诱导叶片对稻瘟病菌的抗性的实例。我们进一步描述了Aubruce-T。钩虫相互作用，以显示来自GD 12的麸皮提取物和N-乙酰基-β-D-氨基葡萄糖苷酶缺陷突变体以浓度依赖性方式差异性地促进生长，并且这些差异与小分子分泌组的差异相关。我们发现，GD 12 mycoparasitises一系列的分离株的出苗前土壤病原体核盘菌，这种相互作用诱导进一步增加植物生长促进以上赋予的GD 12。为了了解T. hamatum GD 12，并促进其作为模式有益生物的使用，以研究植物生长促进，诱导系统抗性和重寄生，我们提出了从头基因组序列数据。我们将GD 12与其他已发表的木霉基因组进行了比较，结果表明，T.钩虫GD 12含有独特的基因组区域，具有编码新的生物活性代谢物的潜力，这些代谢物可能有助于GD 12的农业化学重要性状。
Trichoderma hamatum strain GD12 is unique in that it can promote plant growth, activate biocontrol against pre- and post-emergence soil pathogens and can induce systemic resistance to foliar pathogens. This study extends previous work in lettuce to demonstrate that GD12 can confer beneficial agronomic traits to other plants, providing examples of plant growth promotion in the model dicot, Arabidopsis thaliana and induced foliar resistance to Magnaporthe oryzae in the model monocot rice. We further characterize the lettuce-T. hamatum interaction to show that bran extracts from GD12 and an N-acetyl-β-D-glucosamindase-deficient mutant differentially promote growth in a concentration dependent manner, and these differences correlate with differences in the small molecule secretome. We show that GD12 mycoparasitises a range of isolates of the pre-emergence soil pathogen Sclerotinia sclerotiorum and that this interaction induces a further increase in plant growth promotion above that conferred by GD12. To understand the genetic potential encoded by T. hamatum GD12 and to facilitate its use as a model beneficial organism to study plant growth promotion, induced systemic resistance and mycoparasitism we present de novo genome sequence data. We compare GD12 with other published Trichoderma genomes and show that T. hamatum GD12 contains unique genomic regions with the potential to encode novel bioactive metabolites that may contribute to GD12's agrochemically important traits.
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