5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression.
5mC modification orchestrates choriogenesis and fertilization by preventing prolonged ftz-f1 expression.
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5 mC修饰通过阻止延长的ftz-f1表达来协调绒毛膜发生和受精。
DOI：
10.1038/s41467-023-43987-5
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发表时间：
2023-12-12
影响因子：
16.6
通讯作者：
Ren, Chonghua
中科院分区：
文献类型：
--
作者：
Zhao, Zheng;Li, Liang;Zeng, Ruichen;Lin, Liangguan;Yuan, Dongwei;Wen, Yejie;Li, Na;Cui, Yingying;Zhu, Shiming;Zhang, Zhi-Min;Li, Sheng;Ren, Chonghua
DNA胞嘧啶第5位甲基化（5-methylcytosine，5 mC）是调控基因表达的一种重要表观遗传修饰，但对它如何调控昆虫基因表达知之甚少。在这里，我们追求详细的分子机制，DNMT 1介导的5 mC维护调节女性生殖的德国蟑螂，德国小蠊。我们的研究结果表明，Dnmt 1敲低降低了卵巢中5 mC的水平，在绒毛膜发生过程中上调了许多基因，特别是转录因子ftz-f1。ftz-f1启动子区的低甲基化增加并抑制了绒毛膜发生过程中卵泡细胞中ftz-f1的表达，从而导致异常高水平的20-羟基蜕皮激素并过度上调细胞外基质重塑基因Mmp 1。这些变化通过引起卵泡细胞失巢凋亡、绒毛膜蛋白缺乏和海绵样体畸形进一步损害绒毛膜发生并破坏受精。这项研究大大推进了我们对DNA 5 mC修饰如何调节昆虫雌性生殖的理解。DNA甲基化调控昆虫雌性生殖的机制在很大程度上是未知的。Zhao等人证明，5 mC修饰通过阻止德国小蠊中ftz-f1的长期表达来协调及时的绒毛膜发生和适当的受精。
DNA methylation at the fifth position of cytosine (5-methylcytosine, 5mC) is a crucial epigenetic modification for regulating gene expression, but little is known about how it regulates gene expression in insects. Here, we pursue the detailed molecular mechanism by which DNMT1-mediated 5mC maintenance regulates female reproduction in the German cockroach, Blattella germanica. Our results show that Dnmt1 knockdown decreases the level of 5mC in the ovary, upregulating numerous genes during choriogenesis, especially the transcription factor ftz-f1. The hypomethylation at the ftz-f1 promoter region increases and prolongs ftz-f1 expression in ovarian follicle cells during choriogenesis, which consequently causes aberrantly high levels of 20-hydroxyecdysone and excessively upregulates the extracellular matrix remodeling gene Mmp1. These changes further impair choriogenesis and disrupt fertilization by causing anoikis of the follicle cells, a shortage of chorion proteins, and malformation of the sponge-like bodies. This study significantly advances our understanding of how DNA 5mC modification regulates female reproduction in insects. The mechanism by which DNA methylation regulates female reproduction in insects is largely unknown. Here Zhao et al. demonstrate that the 5mC modification orchestrates timely choriogenesis and proper fertilization by preventing prolonged ftz-f1 expression in the German cockroach.
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影响因子：
3.7
作者：
Irles P;Bellés X;Piulachs MD
通讯作者：
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DOI：
10.1073/pnas.1716897115
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作者：
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影响因子：
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作者：
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通讯作者：
Zhan, Shuai
DOI：
10.1073/pnas.1614383114
发表时间：
2017-01-24
影响因子：
11.1
作者：
Knapp, Elizabeth;Sun, Jianjun
通讯作者：
Sun, Jianjun