High level of somatic mutations detected in a diploid banana wild relative Musa basjoo
High level of somatic mutations detected in a diploid banana wild relative Musa basjoo
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在二倍体香蕉野生近缘种 Musa basjoo 中检测到高水平的体细胞突变
DOI：
10.1007/s00438-022-01959-2
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发表时间：
2022-10
影响因子：
3.1
通讯作者：
Huang Ju
中科院分区：
文献类型：
--
作者：
Ji Yilun;Chen Xiaonan;Lin Shengqiu;Milton Brian Traw;Tian Dacheng;Yang Sihai;Wang Long;Huang Ju
植物被认为缺乏早期分离的生殖系，并且通常保留无性繁殖和有性繁殖，这两者都可以允许体细胞突变进入配子或克隆后代，从而影响植物进化。目前还不清楚这些体细胞突变在植物发育过程中发生的频率，以及有多大比例会传递给它们的有性后代或克隆后代。无性“无核”繁殖在许多果树育种中发挥了重要作用，如柑橘、葡萄和香蕉。这些谱系中的植物是否经历大量的体细胞突变积累是未知的。为了评估植物克隆群体的体细胞突变积累和遗传，本文利用30个来自叶、鞘、穗、根和地下根茎5个结构的全基因组重测序样本，评估了二倍体香蕉野生近缘种Musa basjoo的体细胞突变积累。在每两个相邻的无性系吸盘之间平均观察到18.5个高比例的从头体细胞突变，相当于每无性世代每个位点约2.48 × 10- 8，高于拟南芥和桃的每有性世代每个位点的突变率（< 1 × 10-8）。有趣的是，这些分株间体细胞突变中的大多数同时在同一个体的不同组织中共享，具有高水平的变体等位基因分数，这表明这些体细胞突变在分株发育的早期出现，并且每个个体可能仅从少数顶端干细胞发育。因此，这些结果表明香蕉的野生亲戚中有大量的突变积累。本研究揭示了体细胞突变在芭蕉遗传变异中的重要意义，为香蕉等无性系作物的性状改良育种提供了理论依据。
Plants are thought to lack an early segregating germline and often retain both asexual and sexual reproduction, both of which may allow somatic mutations to enter the gametes or clonal progeny, and thereby impact plant evolution. It is yet unclear how often these somatic mutations occur during plant development and what proportion is transmitted to their sexual or cloned offspring. Asexual "seedless" propagation has contributed greatly to the breeding in many fruit crops, such as citrus, grapes and bananas. Whether plants in these lineages experience substantial somatic mutation accumulation is unknown. To estimate the somatic mutation accumulation and inheritance among a clonal population of plant, here we assess somatic mutation accumulation in Musa basjoo, a diploid banana wild relative, using 30 whole-genome resequenced samples collected from five structures, including leaves, sheaths, panicle, roots and underground rhizome connecting three clonal individuals. We observed 18.5 high proportion de novo somatic mutations on average between each two adjacent clonal suckers, equivalent to ~ 2.48 × 10-8per site per asexual generation, higher than the per site per sexual generation rates (< 1 × 10-8) reported in Arabidopsis and peach. Interestingly, most of these inter-ramet somatic mutations were shared simultaneously in different tissues of the same individual with a high level of variant allele fractions, suggesting that these somatic mutations arise early in ramet development and that each individual may develop only from a few apical stem cells. These results thus suggest substantial mutation accumulation in a wild relative of banana. Our work reveals the significance of somatic mutation in Musa basjoo genetics variations and contribute to the trait improvement breeding of bananas and other asexual clonal crops.
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影响因子：
4.1
作者：
D. Simberloff;Christy Leppanen
通讯作者：
D. Simberloff;Christy Leppanen
影响因子：
5.6
作者：
Wang X;Wang A;Li Y;Xu Y;Wei Q;Wang J;Lin F;Gong D;Liu F;Wang Y;Peng L;Li J
通讯作者：
Li J
DOI：
10.1146/annurev.es.10.110179.000545
发表时间：
1979-06
期刊：
Annual Review of Ecology, Evolution, and Systematics
影响因子：
--
作者：
James White
通讯作者：
James White
影响因子：
64.8
作者：
F. Cramer
通讯作者：
F. Cramer
影响因子：
16.8
作者：
T. Reusch;I. Baums;B. Werner
通讯作者：
T. Reusch;I. Baums;B. Werner