A Novel Banana Mutant "RF 1" (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance.
A Novel Banana Mutant "RF 1" (Musa spp. ABB, Pisang Awak Subgroup) for Improved Agronomic Traits and Enhanced Cold Tolerance and Disease Resistance.
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DOI：
10.3389/fpls.2021.730718
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发表时间：
2021
影响因子：
5.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Wang X;Wang A;Li Y;Xu Y;Wei Q;Wang J;Lin F;Gong D;Liu F;Wang Y;Peng L;Li J
香蕉是一种主要的水果作物，生长在世界各地的热带和亚热带地区。在品种中，“粉椒，FJ”（Musa spp. ABB， Pisang Awak亚群）是一种受欢迎的香蕉品种，因其糖酸混合较好，果实形状相对较小。然而，由于传统的FJ品种生长高度较高，容易倒伏，不适合采收。在这项研究中，我们通过对FJ品种进行甲基磺酸乙酯（EMS）诱变来寻找理想的香蕉突变体。在0.8% (v/v) EMS处理4 h后，我们获得了一个稳定遗传的突变体，这里称为“ReFen 1”（RF1），并观察到半矮化表型。与野生型（FJ）相比，该RF1突变体在中国3个不同地点进行的5年田间试验中表现出持续改善的农艺性状。值得注意的是，RF1植株表现出显著增强的耐寒性和抗斑叶病能力，这主要是由于可溶性糖含量大幅增加、淀粉积累增加以及纤维素沉积减少。因此，这项研究不仅展示了一种强大的遗传策略如何用于水果作物育种，而且为鉴定香蕉等农艺性状改良的新基因提供了见解。
Banana is a major fruit crop grown in tropical and subtropical regions worldwide. Among cultivars, “FenJiao, FJ” (Musa spp. ABB, Pisang Awak subgroup) is a popular variety of bananas, due to its better sugar-acid blend and relatively small fruit shape. However, because the traditional FJ variety grows relatively high in height, it is vulnerable to lodging and unsuitable for harvesting. In this study, we sought desirable banana mutants by carrying out ethyl methanesulfonate (EMS) mutagenesis with the FJ cultivar. After the FJ shoot tips had been treated with 0.8% (v/v) EMS for 4 h, we obtained a stably inherited mutant, here called “ReFen 1” (RF1), and also observed a semi-dwarfing phenotype. Compared with the wild type (FJ), this RF1 mutant featured consistently improved agronomic traits during 5-year field experiments conducted in three distinct locations in China. Notably, the RF1 plants showed significantly enhanced cold tolerance and Sigatoka disease resistance, mainly due to a substantially increased soluble content of sugar and greater starch accumulation along with reduced cellulose deposition. Therefore, this study not only demonstrated how a powerful genetic strategy can be used in fruit crop breeding but also provided insight into the identification of novel genes for agronomic trait improvement in bananas and beyond.
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影响因子：
13.8
作者：
Datta S;Jankowicz-Cieslak J;Nielen S;Ingelbrecht I;Till BJ
通讯作者：
Till BJ
影响因子：
5.6
作者：
Gao R;Guo G;Fang C;Huang S;Chen J;Lu R;Huang J;Fan X;Liu C
通讯作者：
Liu C
影响因子：
3.7
作者：
Amosova, Alexandra, V;Zoshchuk, Svyatoslav A.;Muravenko, Olga, V
通讯作者：
Muravenko, Olga, V
影响因子：
5.6
作者：
Allegra, Alessio;Gallotta, Alessandra;Martinelli, Federico
通讯作者：
Martinelli, Federico
影响因子：
4.9
作者：
Ge, XJ;Liu, MH;Chiang, TY
通讯作者：
Chiang, TY