Behavior variability of Salmonella enterica isolates from meat-related sources
Behavior variability of Salmonella enterica isolates from meat-related sources
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从肉类相关来源分离出的肠道沙门氏菌的行为变异性
DOI：
10.1016/j.lwt.2016.06.036
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发表时间：
2016-11
影响因子：
6
通讯作者：
Zhou Guanghong
中科院分区：
文献类型：
--
作者：
Wang Huhu;Jiang Yun;Liu Xuan;Qian Wenjuan;Xu Xinglian;Zhou Guanghong
肠沙门氏菌因其对食品行业的严重污染和对人类健康的严重威胁而受到全球关注。在这项研究中，评估了来自肉类相关来源的 17 种肠沙门氏菌分离株的生长多样性、基因型毒力和成簇规则间隔短回文重复 (CRISPR) 位点。结果表明，供试菌株的生长动力学行为表现出显着差异（p < 0.05），生长速率与血清型无关，并且在 Sinstorf 血清型中观察到快速生长模式。涉及 6 个毒力单位的 9 个基因的倍数变化显示出分离株之间的差异 (p < 0.05)，与 SPI-2 和 SPI-4 相关的毒力基因表现出高表达。 Sinstorf、Stanley和Aberdeen血清型中被测基因的表达水平明显较低。在测试分离株中，甚至在血清型内，也观察到 CRISPR-1 和 CRISPR-2 的重复长度和间隔数存在相当高的多样性，这也得到了系统发育关系的证实。未观察到每个测试基因的表达水平与 CRISPR 空间数量之间存在显着关系。这些信息表明，沙门氏菌分离株之间在行为方面的变异性很大，对菌株变异性的评估是必不可少的。
Salmonella enterica has been concerned globally due to its serious contamination in the food industry and serious risks to human health. In this study, the diversity of growth, genotypic virulence and clustered regularly interspaced short palindromic repeats (CRISPR) locus of 17 Salmonella enterica isolates from meat-related sources was evaluated. The results showed that the tested isolates exhibited significant differences (p < 0.05) in the growth kinetic behaviors, the growth rate was independent on serotype, and the fast-growing pattern was observed in Sinstorf serotype. The fold-change of nine genes involved in six virulence units showed isolate-to-isolate variation (p < 0.05), virulence genes associated with SPI-2 and SPI-4 displayed high expression. Relative lower expression levels of tested genes were obviously found in serotypes of Sinstorf, Stanley and Aberdeen. A considerable high diversity in repeats length and space numbers of both CRISPR-1 and CRISPR-2 among tested isolates was also observed, even within serotypes, which was also confirmed by the phylogenetic relationships. No significant relationship between the expression levels of each tested gene and the numbers of CRISPR spaces was observed. The information indicated that the variability among isolates ofSalmonellawith respect to their behavioral aspects is extensive, and assessment of strain variability is quite indispensable.
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影响因子：
2.1
作者：
He, Xinlong;Ahn, Juhee
通讯作者：
Ahn, Juhee
影响因子：
2.8
作者：
D. Shah;Xiaohui Zhou;Tarek Addwebi;M. Davis;D. Call
通讯作者：
D. Shah;Xiaohui Zhou;Tarek Addwebi;M. Davis;D. Call
影响因子：
3.7
作者：
Fabre L;Zhang J;Guigon G;Le Hello S;Guibert V;Accou-Demartin M;de Romans S;Lim C;Roux C;Passet V;Diancourt L;Guibourdenche M;Issenhuth-Jeanjean S;Achtman M;Brisse S;Sola C;Weill FX
通讯作者：
Weill FX
影响因子：
3.3
作者：
Hoffmann M;Zhao S;Pettengill J;Luo Y;Monday SR;Abbott J;Ayers SL;Cinar HN;Muruvanda T;Li C;Allard MW;Whichard J;Meng J;Brown EW;McDermott PF
通讯作者：
McDermott PF
影响因子：
2.7
作者：
Pettengill JB;Timme RE;Barrangou R;Toro M;Allard MW;Strain E;Musser SM;Brown EW
通讯作者：
Brown EW