Erythritol triggers expression of virulence traits in Brucella melitensis.
Erythritol triggers expression of virulence traits in Brucella melitensis.
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DOI：
10.1016/j.micinf.2013.02.002
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发表时间：
2013-06
影响因子：
5.8
通讯作者：
Splitter G
中科院分区：
文献类型：
--
作者：
Petersen E;Rajashekara G;Sanakkayala N;Eskra L;Harms J;Splitter G
赤藓糖醇是布鲁氏菌优先利用的四碳糖。山羊、奶牛和猪胎盘中赤藓糖醇的存在被用来解释布鲁氏菌在这些部位的定位以及随后大量细菌的积累，最终导致流产。本研究表明，B. melitensis也会定位于小鼠体内赤藓糖醇的人工位点，为研究布鲁氏菌流产的发病机制提供了一个潜在的模型系统。感染小鼠体内赤藓糖醇部位的免疫组织学染色显示细胞外细菌的比例高于预期。随后的实验表明，在赤藓糖醇存在的情况下，细胞内的B. melitensis无法在巨噬细胞内复制，赤藓糖醇能够到达细胞内细菌的位置。细胞内对生长的抑制被发现鼓励细菌在细胞外而不是细胞内复制，这是布鲁氏菌发病机制中一个特别有趣的发展。为了确定赤藓糖醇对山羊芽孢杆菌基因表达的影响，用微阵列分析了在加赤藓糖醇和不加赤藓糖醇的情况下生长的细菌。暴露于赤藓糖醇后，两个主要的毒力途径（IV型分泌系统VirB和鞭毛蛋白）被上调，表明赤藓糖醇在毒力中起作用。
Erythritol is a four-carbon sugar preferentially utilized by Brucella spp. The presence of erythritol in the placentas of goats, cows, and pigs has been used to explain the localization of Brucella to these sites and the subsequent accumulation of large amounts of bacteria, eventually leading to abortion. Here we show that B. melitensis will also localize to an artificial site of erythritol within a mouse, providing a potential model system to study the pathogenesis of Brucella abortion. Immunohistological staining of the sites of erythritol within infected mice indicated a higher than expected proportion of extracellular bacteria. Ensuing experiments suggested intracellular B. melitensis was unable to replicate within macrophages in the presence of erythritol and that erythritol was able to reach the site of intracellular bacteria. The intracellular inhibition of growth was found to encourage the bacteria to replicate extracellularly rather than intracellularly, a particularly interesting development in Brucella pathogenesis. To determine the effect of erythritol on expression of B. melitensis genes, bacteria grown either with or without erythritol were analyzed by microarray. Two major virulence pathways were up-regulated in response to exposure to erythritol (the type IV secretion system VirB and flagellar proteins), suggesting a role for erythritol in virulence.
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影响因子：
3.1
作者：
Burkhardt, S;de Bagüés, MPJ;Köhler, S
通讯作者：
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影响因子：
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影响因子：
2.2
作者：
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作者：
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通讯作者：
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