Population biology of Streptococcus pneumoniae in West Africa: multilocus sequence typing of serotypes that exhibit different predisposition to invasive disease and carriage.
Population biology of Streptococcus pneumoniae in West Africa: multilocus sequence typing of serotypes that exhibit different predisposition to invasive disease and carriage.
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DOI：
10.1371/journal.pone.0053925
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Antonio M
中科院分区：
文献类型：
--
作者：
Donkor ES;Adegbola RA;Wren BW;Antonio M
尽管大多数肺炎球菌感染发生在发展中国家，但对发展中国家肺炎链球菌的群体生物学知之甚少。该研究的目的是应用 MLST 来调查西非肺炎链球菌的种群生物学。对来自冈比亚、尼日利亚和加纳等三个西非国家的 73 株侵入性和携带性肺炎链球菌菌株进行了调查。分离株涵盖7个血清型（1、3、5、6A、11、14、23F），并进行了多位点序列分型和抗生素敏感性测试。总体而言，鉴定出 50 种不同的序列类型 (ST)，其中 38% (29) 是新颖的。最常见的 ST 是一种新型克隆 - ST 4012 (6.5%)，一些克隆包括 ST 913、925、1737、2160 和 3310 似乎是研究区域特有的。包括 ST 63 和 ST 4012 在内的两个 ST 与多种血清型相关，表明血清型转换的历史。 ST 63 与血清型 3 和 23F 相关，而 ST 4012 与血清型 6A 和 23 相关。使用严格的 6/7 相同基因座定义的 eBURST 分析将 50 个 ST 分为 5 个克隆复合体和 65 个单克隆，在分离株中表达了高水平的遗传多样性。与其他血清型相比，血清型 1 和 5 分离株似乎更具克隆性。在调查的人群中普遍不存在国际公认的肺炎链球菌抗生素耐药性克隆，唯一确定的多重耐药菌株（1/66）属于肺炎球菌流行病学网络克隆 ST 63。西非的肺炎球菌种群差异很大，侵袭性疾病中常见的血清型（例如血清型 1 和 5）比非洲常见的血清型更有可能是克隆性的。马车。
Little is known about the population biology of Streptococcus pneumoniae in developing countries, although the majority of pneumococcal infections occur in this setting. The aim of the study was to apply MLST to investigate the population biology of S. pneumoniae in West Africa. Seventy three invasive and carriage S. pneumoniae isolates from three West African countries including The Gambia, Nigeria and Ghana were investigated. The isolates covered seven serotypes (1, 3, 5, 6A, 11, 14, 23F) and were subjected to multilocus sequence typing and antibiotic susceptibility testing. Overall, 50 different sequence types (STs) were identified, of which 38% (29) were novel. The most common ST was a novel clone-ST 4012 (6.5%), and some clones including STs 913, 925, 1737, 2160 and 3310 appeared to be specific to the study region. Two STs including ST 63 and ST 4012 were associated with multiple serotypes indicating a history of serotype switching. ST 63 was associated with serotypes 3 and 23F, while ST 4012 was associated with serotypes 6A and 23. eBURST analyses using the stringent 6/7 identical loci definition grouped the 50 STs into 5 clonal complexes and 65 singletons, expressing a high level of genetic diversity among the isolates. Compared to the other serotypes, serotypes 1 and 5 isolates appeared to be more clonal. Internationally recognized antibiotic resistant clones of S. pneumoniae were generally absent in the population investigated and the only multidrug resistant isolate identified (1/66) belong to the Pneumocococcal Epidemiology Network clone ST 63. The pneumococcal population in West Africa is quite divergent, and serotypes that are common in invasive disease (such as serotypes 1 and 5) are more likely to be clonal than serotypes that are common in carriage.
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影响因子：
9.4
作者：
Brueggemann, AB;Spratt, BG
通讯作者：
Spratt, BG
DOI：
10.1099/00221287-144-11-3049
发表时间：
1998-11-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Enright, MC;Spratt, BG
通讯作者：
Spratt, BG
影响因子：
6.4
作者：
Donkor ES;Bishop CJ;Gould K;Hinds J;Antonio M;Wren B;Hanage WP
通讯作者：
Hanage WP
影响因子：
4.2
作者：
Antonio, Martin;Hakeem, Ishrat;Adegbola, Richard A.
通讯作者：
Adegbola, Richard A.
影响因子：
2.6
作者：
Blossom, D. B.;Cordeiro, S. M.;Jacobs, M. R.
通讯作者：
Jacobs, M. R.