Using rootstock to increase watermelon fruit yield and quality at low potassium supply: A comprehensive analysis from agronomic, physiological and transcriptional perspective
Using rootstock to increase watermelon fruit yield and quality at low potassium supply: A comprehensive analysis from agronomic, physiological and transcriptional perspective
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低钾供应下利用砧木提高西瓜果实产量和品质：农艺、生理和转录角度的综合分析
DOI：
10.1016/j.scienta.2018.06.091
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发表时间：
2018-11
影响因子：
4.3
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Zhong YQ;Chen C;Nawaz MA;Jiao YY;Zheng ZH;Shi XF;Xie WY;Yu YG;Guo J;Zhu SH;Xie M;Kong QS;Cheng F;Bie ZL;Huang Y
嫁接是西瓜生产中广泛应用的技术。低钾条件下嫁接对西瓜产量和品质的影响及其机理尚不清楚。本研究从农艺学、生理学和转录学的角度探讨了这一问题。西瓜[Citrullus lanatus（Thunb.）马特森和Nakai，cv.早佳8424]，采用自接或嫁接于砧木“永实”（Citrullus lanatussp.）“京新珍1号”（Lagenaria siceraria Standl.）和“清岩珍1号”（Cucurbita maxima × C. Moschata）。将植物置于6.0 mM K（正常K）和0.1 mM K（低K）下。与6.0 mM K处理相比，0.1 mM K处理的果实产量较低，表现为单果重下降，但“永实”、“京新珍1号”和“青岩珍1号”嫁接的果实产量下降幅度较小（分别为10%、15%和19%），而自接的果实产量下降幅度较小（38%）。在0.1mM K处理下，砧木嫁接植株茎、叶、果皮和果肉中K+浓度明显高于自接植株。此外，砧木嫁接植株叶片玉米素核苷和叶绿素含量显著高于砧木嫁接植株. 0.1 mM K处理显著降低了自接植株的可溶性固形物、蔗糖、维生素C、番茄红素、β-胡萝卜素等品质。然而，0.1 mM K处理并没有导致砧木嫁接植株中大多数测定的果实品质参数的明显下降。果实转录组分析结果表明，自接、“永实”、“京新珍1号”和“青岩珍1号”嫁接植株中分别有670、27、16和15个对0.1mM钾敏感的差异表达基因，表明砧木嫁接降低了西瓜果实对0.1mM钾的敏感性，GO和KEGG分析表明，大部分DGE在细胞代谢过程和代谢途径中富集。低钾（0.1 mM K）处理显著提高了钾通道基因Cla 020934的表达，但降低了番茄红素合成酶基因Cla 009122的表达。综上所述，与自接植株相比，砧木嫁接西瓜可提高植株果实产量和品质，降低果肉转录组对低钾的敏感性。并对低钾条件下提高性能的机理进行了探讨。
Grafting is a widely used technique in watermelon production. How grafting affects watermelon fruit yield and quality at low potassium supply and the mechanism remains unclear. This study addresses the question from agronomic, physiological and transcriptional perspective. Watermelon plants [Citrullus lanatus(Thunb.) Matsum. and Nakai, cv. Zaojia 8424], either self-grafted or grafted onto the rootstock ‘Yongshi’ (Citrullus lanatussp.), ‘Jingxinzhen No.1’ (Lagenaria sicerariaStandl.), and ‘Qingyanzhen No.1’ (Cucurbita maxima × C. moschata). Plants were subjected to 6.0 mM K (normal K) and 0.1 mM K (low K). Compared with plants treated with 6.0 mM K, those supplied with 0.1 mM K produced less fruit yield as indicated by the decrease in single fruit weight of all plants; however, a smaller decrease was observed in plants grafted onto ‘Yongshi’ (10%), ‘Jingxinzhen No.1’ (15%) and ‘Qingyanzhen No.1’ (19%) than the self- grafted watermelon (38%). The K+concentration of stem, leaf, fruit peel and flesh were obviously higher in the rootstock- grafted plants than in the self- grafted ones under 0.1 mM K. In addition, rootstock- grafted plant had significantly higher leaf zeatin riboside and chlorophyll content. Fruit quality, including contents of total soluble solid, sucrose, vitamin C, lycopene, β-carotene, were significantly decreased in the self- grafted plants under 0.1 mM K. However, 0.1 mM K treatment did not result in an obvious decrease in most of the measured fruit quality parameters in the rootstock- grafted plants. Fruit transcriptome analysis showed that there were 670, 27, 16 and 15 differential expressed genes (DGEs) responded to 0.1 mM K in the self- grafted, ‘Yongshi’, ‘Jingxinzhen No.1’ and ‘Qingyanzhen No.1’- grafted plants, respectively, indicating that rootstock grafting decrease the sensitivity of watermelon fruit to 0.1 mM K at the transcriptome level, GO and KEGG analysis showed that most of the DGEs were enriched in cellular metabolic process and metabolic pathways. Low K (0.1 mM K) significantly increased the gene expression of potassium channel (Cla020934), but decreased the gene expression of phytoene synthase (Cla009122-responsible for lycopene synthesis) in the fruit flesh of self-grafted watermelon. Taken together, the above results suggested that compared with self- grafted plants, watermelon grafted onto rootstock can enhance plant fruit yield, quality and decrease the sensitivity of fruit flesh transcriptome to low K. The mechanism of improved performance under low potassium is discussed.
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影响因子：
2.5
作者：
Hao Yan-shu;W. Xiaoli;Xiaocheng Ying;Wu Li-shu;Jiang Cuncang
通讯作者：
Hao Yan-shu;W. Xiaoli;Xiaocheng Ying;Wu Li-shu;Jiang Cuncang
影响因子：
4.6
作者：
Zhang X;Jiang H;Wang H;Cui J;Wang J;Hu J;Guo L;Qian Q;Xue D
通讯作者：
Xue D
影响因子：
2.5
作者：
M. Fan;Z. Bie;Huiying Xie;Fang Zhang;Shuang Zhao;Hongyan Zhang
通讯作者：
M. Fan;Z. Bie;Huiying Xie;Fang Zhang;Shuang Zhao;Hongyan Zhang
两种对比西瓜基因型钾饥饿反应的比较转录组分析
影响因子：
4.3
作者：
Fan, Molin;Huang, Yuan;Bie, Zhilong
通讯作者：
Bie, Zhilong
影响因子：
1.9
作者：
Colla, Giuseppe;Suarez, Carolina Maria Cardona;Rouphael, Youssef
通讯作者：
Rouphael, Youssef