Minimal components of the RNA polymerase II transcription apparatus determine the consensus TATA box.
Minimal components of the RNA polymerase II transcription apparatus determine the consensus TATA box.
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DOI：
10.1093/nar/gkn130
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发表时间：
2008-05
影响因子：
14.9
通讯作者：
Myers LC
中科院分区：
文献类型：
--
作者：
Bjornsdottir G;Myers LC
在酿酒酵母中，多种方法已经达到TATA（T/A）A（A/T）（A/G）的共有TATA盒序列。TATA结合蛋白（TBP）的亲和力本身并不能决定TATA盒的功能。为了发现如何一组最小的基础和激活的转录所需的因素有助于一个功能性的TATA框的序列要求，我们进行了转录反应，使用高度纯化的蛋白质和CYC 1启动子TATA框突变体。TATA盒共有序列是启动子活性的良好预测因子。然而，几个非共识序列几乎是完全功能性的，这表明机械要求不是TATA盒上唯一的选择压力。我们还发现，在某个位置的突变的影响往往取决于其他碱基在一个特定的TATA框。虽然激活子和辅激活子强烈影响TBP募集和启动子的稳定性，但无论是介体、激活子Gal 4-V16还是TFIID都不能特异性地补偿弱TATA盒的低转录水平。然而，在纯化的转录反应中加入介体确实增加了对某些共有TATA序列的功能选择性。在全细胞提取物或在体内与这些TATA盒突变体的转录表明，因子，而不是在我们的纯化系统，可能有助于启动弱TATA盒转录。
In Saccharomyces cerevisiae, multiple approaches have arrived at a consensus TATA box sequence of TATA(T/A)A(A/T)(A/G). TATA-binding protein (TBP) affinity alone does not determine TATA box function. To discover how a minimal set of factors required for basal and activated transcription contributed to the sequence requirements for a functional TATA box, we performed transcription reactions using highly purified proteins and CYC1 promoter TATA box mutants. The TATA box consensus sequence is a good predictor of promoter activity. However, several nonconsensus sequences are almost fully functional, indicating that mechanistic requirements are not the only selective pressure on the TATA box. We also found that the effect of a mutation at a certain position is often dependent on other bases within a particular TATA box. Although activators and coactivators strongly influence TBP recruitment and stability at promoters, neither Mediator, the activator Gal4-V16, nor TFIID specifically compensate for the low transcription levels of the weak TATA boxes. The addition of Mediator to purified transcription reactions did, however, increase the functional selectivity for certain consensus TATA sequences. Transcription in whole-cell extracts or in vivo with these TATA box mutants indicated that factors, other than those in our purified system, may help initiate transcription from weak TATA boxes.
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影响因子：
5.3
作者：
HARBURY, PAB;STRUHL, K
通讯作者：
STRUHL, K
影响因子：
64.5
作者：
Basehoar, AD;Zanton, SJ;Pugh, BF
通讯作者：
Pugh, BF
DOI：
10.1073/pnas.86.15.5718
发表时间：
1989-08-01
影响因子：
11.1
作者：
HAHN, S;BURATOWSKI, S;GUARENTE, L
通讯作者：
GUARENTE, L
影响因子：
64.8
作者：
Lee, TI;Causton, HC;Young, RA
通讯作者：
Young, RA
影响因子：
64.8
作者：
KIM, JL;NIKOLOV, DB;BURLEY, SK
通讯作者：
BURLEY, SK