Perineural pretreatment of bee venom attenuated the development of allodynia in the spinal nerve ligation injured neuropathic pain model; an experimental study.
Perineural pretreatment of bee venom attenuated the development of allodynia in the spinal nerve ligation injured neuropathic pain model; an experimental study.
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DOI：
10.1186/1472-6882-14-431
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发表时间：
2014-11-04
影响因子：
--
通讯作者：
Shin JW
中科院分区：
文献类型：
--
作者：
Koh WU;Choi SS;Lee JH;Lee SH;Lee SK;Lee YK;Leem JG;Song JG;Shin JW
已知稀释的蜂毒（BV）具有抗伤害感受和抗炎作用。因此，我们评估了神经周围蜂毒预处理是否可以减轻脊神经结扎损伤动物模型中神经病理性疼痛的发展。通过结扎L5和L 6脊神经，在30只雄性Sprague道利大鼠中手术诱导神经病理性疼痛，其中各10只大鼠用盐水和0.05和0.1 mg BV处理。在术后第3 - 29天进行机械、冷和热异常性疼痛的行为测试。第9天每组处死3只大鼠和9只假手术大鼠，分析同侧L5背根神经节中瞬时受体电位香草酸1型（TRPV 1）、锚蛋白1型（TRPA 1）和melastatin 8型（TRPM 8）受体的表达。BV神经周给药脊髓神经减弱了机械，热和冷异常性疼痛的发展，BV预处理减少了同侧背根神经节中TRPV 1，TRPA 1，TRPM 8和c-Fos的表达。目前的研究表明，在脊神经结扎诱导前，用稀释的蜂毒进行神经周围预处理可以显著抑制神经病理性疼痛的发展。此外，这种蜂毒诱导的抑制与瞬时受体电位家族成员的参与密切相关。
Diluted bee venom (BV) is known to have anti-nociceptive and anti-inflammatory effects. We therefore assessed whether perineural bee venom pretreatment could attenuate the development of neuropathic pain in the spinal nerve ligation injured animal model. Neuropathic pain was surgically induced in 30 male Sprague Dawley rats by ligation of the L5 and L6 spinal nerves, with 10 rats each treated with saline and 0.05 and 0.1 mg BV. Behavioral testing for mechanical, cold, and thermal allodynia was conducted on postoperative days 3 to 29. Three rats in each group and 9 sham operated rats were sacrificed on day 9, and the expression of transient receptor potential vanilloid type 1 (TRPV1), ankyrin type 1 (TRPA1), and melastatin type 8 (TRPM8) receptors in the ipsilateral L5 dorsal root ganglion was analyzed. The perineural administration of BV to the spinal nerves attenuated the development of mechanical, thermal, and cold allodynia, and the BV pretreatment reduced the expression of TRPV1, TRPA1, TRPM8 and c − Fos in the ipsilateral dorsal root ganglion. The current study demonstrates that the perineural pretreatment with diluted bee venom before the induction of spinal nerve ligation significantly suppresses the development of neuropathic pain. Furthermore, this bee venom induced suppression was strongly related with the involvement of transient receptor potential family members.
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DOI：
10.1016/j.bbrc.2007.05.029
发表时间：
2007-07-13
影响因子：
3.1
作者：
Frederick, J.;Buck, M. E.;Cortright, D. N.
通讯作者：
Cortright, D. N.
影响因子：
16.2
作者：
Kwan, KY;Allchorne, AJ;Corey, DP
通讯作者：
Corey, DP
影响因子：
5.4
作者：
Freire Saad Rached, Izabella Cordeiro;Castro, Fabio Morato;Verissimo de Mello, Suzana Beatriz
通讯作者：
Verissimo de Mello, Suzana Beatriz
影响因子：
7.4
作者：
Kwon, YB;Lee, JD;Lee, JH
通讯作者：
Lee, JH
影响因子：
7.4
作者：
Knowlton WM;Bifolck-Fisher A;Bautista DM;McKemy DD
通讯作者：
McKemy DD