Comparison of the Oral Microbiota Structure among People from the Same Ethnic Group Living in Different Environments.
Comparison of the Oral Microbiota Structure among People from the Same Ethnic Group Living in Different Environments.
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DOI：
10.1155/2022/6544497
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发表时间：
2022
影响因子：
--
通讯作者：
Li, Yongming
中科院分区：
文献类型：
--
作者：
Ma, Guoyun;Qiao, Yanan;Shi, Han;Zhou, Jianye;Li, Yongming
口腔微生物群的特征可能取决于口腔健康、年龄、饮食和地理，但地理环境对口腔微生物群的影响受到的关注有限。据报道，口腔微生物群的特征在城市和农村环境之间存在差异。为了尽量减少遗传背景的影响，我们招募了54名来自同一民族的志愿者，居住在中国甘肃省的城市和农村地区。我们收集了牙菌斑样本，并根据参与者的居住地区和龋齿状况将其分为四组。我们使用Pacific Biosciences测序平台对这些样本的16S rRNA进行测序，并分析了地理区域与口腔微生物群特征之间的相关性。α和β多样性分析显示，四组间牙菌斑菌群的多样性和组成存在显著差异。聚类分析表明，地理区域在决定口腔微生物群中起着重要作用。口腔微生物网络分析表明，地理差异对口腔微生物的组成特征和内部结构有重要影响。我们发现，一些优势菌株，可能发挥关键作用，在维持口腔健康，如口腔链球菌，生痰二氧化碳嗜纤维菌，猫卟啉单胞菌，马图霍棒状杆菌，副流感嗜血杆菌，和普氏普雷沃菌，受地理环境的影响较小。这些结果提供了对影响口腔微生物群组成的因素的更深入了解，并有助于在不同环境中早期诊断和有效预防龋齿。
The characteristics of the oral microbiota may depend on oral health, age, diet, and geography, but the influence of the geographic setting on the oral microbiota has received limited attention. The characteristics of oral microbiota have been reported to differ between urban and rural environments. In order to minimize the influence of genetic background, we recruited 54 volunteers from the same ethnic group, living in urban and rural areas of Gansu Province, China. We collected dental plaque samples and divided them into four groups according to the participant's area of residence and dental caries status. We sequenced the 16S rRNA of these samples using the Pacific Biosciences sequencing platform and analyzed the correlation between the geographic area and the characteristics of the oral microbiota. Analysis of the alpha and beta diversity revealed that there were significant differences in diversity and composition of dental plaque microflora among the four groups. Cluster analysis revealed that geographic area played an important role in determining the oral microbiota. Network analysis of oral microorganisms showed that geographic differences had major influence on the composition characteristics and internal structure of oral microorganisms. We found that some dominant strains which may play a key role in maintaining oral health, such as Streptococcus oralis, Capnocytophaga sputigena, Porphyromonas catoniae, Corynebacterium matruchotii, Haemophilus parainfluenzae, and Prevotella loescheii, were less affected by the geographic setting. These results provide a deeper understanding of factors influencing the composition of the oral microbiota and could contribute to early diagnosis and effective prevention of dental caries in different settings.
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Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11.8
作者：
BILGRAMI, S;BERGSTROM, SK;ASCENSAO, JL
通讯作者：
ASCENSAO, JL
影响因子：
5.2
作者：
Bouillaguet S;Manoil D;Girard M;Louis J;Gaïa N;Leo S;Schrenzel J;Lazarevic V
通讯作者：
Lazarevic V
影响因子：
2.7
作者：
Crielaard W;Zaura E;Schuller AA;Huse SM;Montijn RC;Keijser BJ
通讯作者：
Keijser BJ
影响因子：
6.7
作者：
Doungudomdacha, S;Rawlinson, A;Douglas, CWI
通讯作者：
Douglas, CWI