Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum.
Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum.
复制标题
DOI：
10.1186/1477-7827-8-152
复制
发表时间：
2010-12-22
期刊：
影响因子：
--
通讯作者：
Heuwieser W
中科院分区：
文献类型：
--
作者：
Gabler C;Fischer C;Drillich M;Einspanier R;Heuwieser W
子宫壁的炎症过程和感染必须被接受为产犊后奶牛的生理事件。这可能会导致临床或亚临床子宫内膜炎，这被认为会损害当前哺乳期的生殖能力。一些细胞因子和急性时相蛋白已被讨论为这些炎症过程的局部和全身介质。本研究旨在探讨子宫内膜趋化因子CXC配体5（CXCL 5）、白细胞介素1β（IL 1B）、IL 6、IL 8、肿瘤坏死因子α（TNF）、结合珠蛋白（HP）、胰高血糖素内过氧化物合成酶2（PTGS 2）mRNA在产后子宫内膜中的表达。使用细胞刷技术，在产后（pp）第10、17、24、31、38和45天从经产奶牛（n = 5）中获得子宫内膜样本。从细胞刷样本制备细胞学涂片，以确定多形核中性粒细胞（PMN）的比例。从子宫内膜标本中提取总RNA，并进行实时RT-PCR。一个时间依赖性的mRNA表达的调查因素被发现的过程中的产后期。详细地，与第31天pp相比，在第17天pp观察到这些因子的显著更高表达。此外，PMN的比例在pp 10-24天之间达到峰值，此后在pp 31天及之后下降至低百分比（< 5%）。此外，CXCL 5、IL 1B、IL 8和HP mRNA表达与PMN比例显著相关（P < 0.05）。炎症子宫内膜组织中CXCL 5、IL 1B、IL 6、IL 8、PTGS 2和TNF mRNA的含量显著高于健康子宫内膜组织（P < 0.05）。这些结果表明，炎症细胞因子和急性时相蛋白在产后期间以时间相关的方式在牛子宫内膜中表达，其中在第17天pp具有显著的表达峰值，这可能是子宫中的粘膜免疫应答。对这些候选基因的表达模式的评估可能比仅确定PMN的百分比来判断炎症的严重性揭示更多的信息。
Inflammatory processes and infections of the uterine wall must be accepted as a physiological event in dairy cows after calving. This might result in clinical or subclinical endometritis which is assumed to impair reproductive performance in the current lactation. Several cytokines and acute phase proteins have been discussed as local and systemic mediators of these inflammatory processes. The aim of the present study was to investigate the endometrial mRNA expression of the chemokine CXC ligand 5 (CXCL5), interleukin 1β (IL1B), IL6, IL8, tumour necrosis factor alpha (TNF), prostaglandin-endoperoxide synthase 2 (PTGS2) and haptoglobin (HP) in the postpartum period. Endometrial samples were obtained from primiparous cows (n = 5) on days 10, 17, 24, 31, 38 and 45 postpartum (pp) using the cytobrush technique. Cytological smears were prepared from cytobrush samples to determine the proportion of polymorphonuclear neutrophils (PMN). Total RNA was extracted from endometrial samples, and real-time RT-PCR was performed. A time-dependent mRNA expression of the investigated factors was found for the course of the postpartum period. In detail, a significantly higher expression of these factors was observed on day 17 pp compared to day 31 pp. Furthermore, the proportion of PMN peaked between days 10-24 pp and decreased thereafter to low percentages (< 5%) on day 31 pp and thereafter. In addition, CXCL5, IL1B, IL8 and HP mRNA expression correlated significantly with the proportion of PMN (P < 0.05). A significantly higher CXCL5, IL1B, IL6, IL8, PTGS2 and TNF mRNA content was observed in samples from cows with an inflamed endometrium compared with samples from cows with a healthy endometrium (P < 0.05). These results show that inflammatory cytokines and acute phase proteins are expressed in the bovine endometrium in a time-related manner during the postpartum period, with a significant expression peak on day 17 pp as a possible mucosal immune response in the uterus. The evaluation of the expression patterns of such candidate genes may reveal more information than only determining the percentage of PMN to judge the severity of an inflammation.
登录
查看更多内容
DOI：
10.1186/1477-7827-7-55
发表时间：
2009-05-29
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Herath S;Lilly ST;Santos NR;Gilbert RO;Goetze L;Bryant CE;White JO;Cronin J;Sheldon IM
通讯作者：
Sheldon IM
影响因子：
3.6
作者：
Sheldon IM;Cronin J;Goetze L;Donofrio G;Schuberth HJ
通讯作者：
Schuberth HJ
影响因子：
2.8
作者：
Barlund, C. S.;Carruthers, T. D.;Palmer, C. W.
通讯作者：
Palmer, C. W.
DOI：
10.1530/jrf.0.0930341
发表时间：
1991-11-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BUTT, BM;SENGER, PL;WIDDERS, PR
通讯作者：
WIDDERS, PR
影响因子：
2.8
作者：
Gautam, G.;Nakao, T.;Hayashi, A.
通讯作者：
Hayashi, A.