Gut commensal microbiota and decreased risk for Enterobacteriaceae bacteriuria and urinary tract infection.
Gut commensal microbiota and decreased risk for Enterobacteriaceae bacteriuria and urinary tract infection.
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DOI：
10.1080/19490976.2020.1805281
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发表时间：
2020-11-09
期刊：
影响因子：
12.2
通讯作者：
Lee JR
中科院分区：
文献类型：
--
作者：
Magruder M;Edusei E;Zhang L;Albakry S;Satlin MJ;Westblade LF;Malha L;Sze C;Lubetzky M;Dadhania DM;Lee JR
尿路感染（UTI）是肾移植受者的常见并发症，可导致显著的发病率和死亡率。最近的证据支持肠道作为UTI来源的作用，但对肠道细菌和UTI发展之间的关系知之甚少。我们假设肠道细菌的丰富与发生菌尿和尿路感染的风险较低有关。我们使用V4-V5高变区的16 S rRNA基因测序对168名肾移植受者的510份粪便标本进行了肠道微生物组分析。51例肾移植受者（30%）在移植后前6个月内发生肠杆菌科菌尿（肠杆菌科菌尿组），117例未发生（无肠杆菌科菌尿组）。无肠杆菌科菌尿组粪便标本中粪杆菌属和Romboutsia的相对丰度显著高于肠杆菌科菌尿组（校正P值<.01）。粪杆菌和Romboutsia的组合相对丰度与肠杆菌科的相对丰度呈负相关（r =-0.13，P = .003）。在多变量考克斯回归分析中，粪杆菌和罗姆布氏菌组合相对丰度的最高三分位数临界值≥13.7%与肠杆菌科菌尿（风险比0.3，P = 0.02）和肠杆菌科UTI（风险比0.4，P = 0.09）风险降低独立相关。总之，我们确定了与肾移植受者中肠杆菌科菌尿症和肠杆菌科UTI风险降低相关的细菌分类群，这支持了未来关于调节肠道微生物群作为预防UTI的新型治疗方法的研究。
Urinary tract infection (UTI) is a common complication in kidney transplant recipients and can lead to significant morbidity and mortality. Recent evidence supports a role for the gut as a source for UTIs but little is known about the relationship between gut commensal bacteria and UTI development. We hypothesized that the abundance of gut commensal bacteria is associated with a lower risk of developing bacteriuria and UTIs. We performed gut microbiome profiling using 16S rRNA gene sequencing of the V4-V5 hypervariable region on 510 fecal specimens in 168 kidney transplant recipients. Fifty-one kidney transplant recipients (30%) developed Enterobacteriaceae bacteriuria within the first 6 months after transplantation (Enterobacteriaceae Bacteriuria Group) and 117 did not (No Enterobacteriaceae Bacteriuria Group). The relative abundances of Faecalibacterium and Romboutsia were significantly higher in the fecal specimens from the No Enterobacteriaceae Bacteriuria Group than those from the Enterobacteriaceae Bacteriuria Group (Adjusted P value<.01). The combined relative abundance of Faecalibacterium and Romboutsia was inversely correlated with the relative abundance of Enterobacteriaceae (r = −0.13, P = .003). In a multivariable Cox Regression, a top tercile cutoff of the combined relative abundance of Faecalibacterium and Romboutsia of ≥13.7% was independently associated with a decreased risk for Enterobacteriaceae bacteriuria (hazard ratio 0.3, P = .02) and Enterobacteriaceae UTI (hazard ratio 0.4, P = .09). In conclusion, we identify bacterial taxa associated with decreased risk for Enterobacteriaceae bacteriuria and Enterobacteriaceae UTI in kidney transplant recipients, which supports future studies on modulating the gut microbiota as a novel treatment for preventing UTIs.
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DOI：
10.1038/nrmicro3432
发表时间：
2015-05
期刊：
Nature reviews. Microbiology
影响因子：
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作者：
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通讯作者：
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影响因子：
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发表时间：
2018-10-01
影响因子：
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作者：
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