Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties.
Calcium-silicate mesoporous nanoparticles loaded with chlorhexidine for both anti- Enterococcus faecalis and mineralization properties.
复制标题
负载洗必太的硅酸钙介孔纳米粒子，具有抗粪肠球菌和矿化特性
DOI：
10.1186/s12951-016-0224-7
复制
发表时间：
2016-10-21
影响因子：
10.2
通讯作者：
Fan B
中科院分区：
文献类型：
--
作者：
Fan W;Li Y;Sun Q;Ma T;Fan B
在感染的根尖周组织中，粪肠球菌是最常见的优势菌之一。洗必泰对大肠杆菌有较强的抗菌作用。但不能促进根尖周围组织的矿化。介孔硅酸钙纳米粒子是一种新型的生物材料，具有良好的促进矿化和可控载放生物活性分子的能力。本研究将氯己定功能化介孔硅酸钙纳米粒子，并对其体外释药、抗菌、细胞增殖和矿化性能进行了评价。通过混合偶联法将洗必泰成功地嵌入到介孔硅酸钙纳米粒子中。新材料可以在不同条件下以碱性pH值持续释放氯己定以及Ca 2+和SiO 3 2-。对大肠杆菌的抑菌能力。在氯己定掺入后，粪便显著改善。含氯己定的纳米粒在低浓度下对细胞增殖无负面影响。在牙本质切片上，新合成的材料表现出类似的抑制E。洗必泰一样。在SBF中浸泡9天后，在材料片的表面上可以观察到大量的磷灰石晶体。负载洗必泰的介孔硅酸钙纳米粒子具有离子和洗必泰的释放、低细胞毒性、优异的抗菌能力和体外矿化能力。该材料可开发成为牙科领域新型有效的根管内药物或用于感染性骨缺损的新型骨缺损填充材料。
In infected periapical tissues, Enterococcus faecalis is one of the most common dominant bacteria. Chlorhexidine has been proved to show strong antibacterial ability against E. faecalis but is ineffective in promoting mineralization for tissues around root apex. Mesoporous calcium-silicate nanoparticles are newly synthesized biomaterials with excellent ability to promote mineralization and carry-release bioactive molecules in a controlled manner. In this study, mesoporous calcium-silicate nanoparticles were functionalized with chlorhexidine and their releasing profile, antibacterial ability, effect on cell proliferation and in vitro mineralization property were evaluated. The chlorhexidine was successfully incorporated into mesoporous calcium-silicate nanoparticles by a mixing-coupling method. The new material could release chlorhexidine as well as Ca2+ and SiO3 2− in a sustained manner with an alkaline pH value under different conditions. The antimicrobial ability against planktonic E. faecalis was dramatically improved after chlorhexidine incorporation. The nanoparticles with chlorhexidine showed no negative effect on cell proliferation with low concentrations. On dentin slices, the new synthesized material demonstrated a similar inhibitory effect on E. faecalis as the chlorhexidine. After being immersed in SBF for 9 days, numerous apatite crystals could be observed on surfaces of the material tablets. Mesoporous calcium-silicate nanoparticles loaded with chlorhexidine exhibited release of ions and chlorhexidine, low cytotoxicity, excellent antibacterial ability and in vitro mineralization. This material could be developed into a new effective intra-canal medication in dentistry or a new bone defect filling material for infected bone defects.
登录
查看更多内容
影响因子：
5
作者：
Carrilho, Marcela R.;Carvalho, Ricardo M.;Sousa, Ethan N.;Nicolau, Jose;Breschi, Lorenzo;Mazzoni, Annalisa;Tjaderhane, Leo;Tay, Franklin R.;Agee, Kelli;Pashley, David H.
通讯作者：
Pashley, David H.
影响因子：
3.2
作者：
Giannelli, M.;Chellini, F.;Tani, A.
通讯作者：
Tani, A.
影响因子：
4.2
作者：
Distel, JW;Hatton, JF;Gillespie, MJ
通讯作者：
Gillespie, MJ
影响因子：
14.9
作者：
Maria Ferrer-Luque, Carmen;Teresa Arias-Moliz, Maria;Ruiz-Linares, Matilde;Martinez Garcia, Maria Elena;Baca, Pilar
通讯作者：
Baca, Pilar
影响因子：
1.9
作者：
Ceci, Monica;Delpech, Gaston;Baldaccini, Beatriz
通讯作者：
Baldaccini, Beatriz