Neural correlates of blood flow measured by ultrasound.
Neural correlates of blood flow measured by ultrasound.
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DOI：
10.1016/j.neuron.2022.02.012
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发表时间：
2022-05-18
期刊：
影响因子：
16.2
通讯作者：
Carandini, Matteo
中科院分区：
文献类型：
--
作者：
Nunez-Elizalde, Anwar O.;Krumin, Michael;Reddy, Charu Bai;Montaldo, Gabriel;Urban, Alan;Harris, Kenneth D.;Carandini, Matteo
功能性超声成像（fUSI）是一种很有吸引力的测量血流量并由此推断大脑活动的方法，但它依赖于神经血管耦合的生理学，并且需要大量的信号处理。为了确定fUSI逐个试验信号反映神经活动的程度，我们在清醒小鼠中使用Neuropixels探针进行了同时fUSI和神经记录。fUSI信号与局部放电率的缓慢（<0.3 Hz）波动密切相关，并通过局部神经元（特别是假定的抑制性神经元）的平滑放电率进行密切预测。最佳平滑滤波器的宽度为10.3 s，与清醒小鼠的血流动力学反应函数相匹配，在小鼠和刺激条件下不变，并且在皮层和海马中相似。fUSI信号在空间上也与神经放电相匹配：两个半球之间的放电率与fUSI信号一样高度相关。因此，通过超声测量的血流与神经元放电具有简单而准确的关系。在小鼠血流的超声成像（fUSI）过程中记录神经活动fUSI信号匹配局部神经元的平滑放电率，特别是抑制性fUSI信号。3s平滑滤波器是清醒小鼠的常规血流动力学反应。放电率的时空相关性在很大程度上解释了血流的相关性。在清醒的小鼠中，用电极同时测量神经活动，用功能超声成像（fUSI）同时测量血流。他们的研究结果表明，血流与频率<0.3 Hz的局部神经元放电之间存在简单而准确的关系，并验证了fUSI作为推断神经活动的工具。
Functional ultrasound imaging (fUSI) is an appealing method for measuring blood flow and thus infer brain activity, but it relies on the physiology of neurovascular coupling and requires extensive signal processing. To establish to what degree fUSI trial-by-trial signals reflect neural activity, we performed simultaneous fUSI and neural recordings with Neuropixels probes in awake mice. fUSI signals strongly correlated with the slow (<0.3 Hz) fluctuations in the local firing rate and were closely predicted by the smoothed firing rate of local neurons, particularly putative inhibitory neurons. The optimal smoothing filter had a width of ∼3 s, matched the hemodynamic response function of awake mice, was invariant across mice and stimulus conditions, and was similar in the cortex and hippocampus. fUSI signals also matched neural firing spatially: firing rates were as highly correlated across hemispheres as fUSI signals. Thus, blood flow measured by ultrasound bears a simple and accurate relationship to neuronal firing. Neural activity was recorded during ultrasound imaging (fUSI) of blood flow in mice fUSI signals matched the smoothed firing rate of local neurons, especially inhibitory The ∼3 s smoothing filter is the stereotypical hemodynamic response of awake mice Spatiotemporal correlations in firing rates largely explain correlations in blood flow Nunez-Elizalde et al. simultaneously measured neural activity with electrodes and blood flow with functional ultrasound imaging (fUSI) in awake mice. Their results establish that blood flow bears a simple and accurate relationship to local neuronal firing at frequencies <0.3 Hz and validate fUSI as a tool to infer neural activity.
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DOI：
10.1177/1073858418805427
发表时间：
2019-08
期刊：
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry
影响因子：
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作者：
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影响因子：
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DOI：
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发表时间：
2012-05-18
期刊：
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影响因子：
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