The feline oral microbiome: a provisional 16S rRNA gene based taxonomy with full-length reference sequences.
The feline oral microbiome: a provisional 16S rRNA gene based taxonomy with full-length reference sequences.
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DOI：
10.1016/j.vetmic.2014.11.019
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发表时间：
2015-02-25
影响因子：
3.3
通讯作者：
Marshall-Jones, Zoe V.
中科院分区：
文献类型：
--
作者：
Dewhirst, Floyd E.;Klein, Erin A.;Bennett, Marie-Louise;Croft, Julie M.;Harris, Stephen J.;Marshall-Jones, Zoe V.
已知人类口腔微生物组在人类健康和疾病中发挥重要作用。虽然研究较少，但猫科动物口腔微生物组被认为起着类似的重要作用。为了确定口腔细菌在健康和疾病中的作用，首先必须能够准确识别存在的细菌种类。16 S rRNA基因序列信息被广泛用于细菌的分子鉴定，也可用于建立新物种的分类。本研究的目的是获得猫口腔细菌的完整16 S rRNA基因参考序列，将序列置于物种水平的细菌分类中，并为常见的猫口腔细菌创建一个基于16 S RNA的分类。克隆库的生产使用“通用”和门选择性PCR引物和DNA从健康和牙周病猫的龈下菌斑池。龈下样本中的细菌也被培养以获得分离株。全长16 S rDNA序列进行了测定的克隆和菌株，代表171猫口腔分类群。根据每个分类单元在16 S rRNA系统发育树中的位置，建立了一个临时的策划分类。猫口腔微生物组策展分类法和16 S rRNA基因参考集将使研究人员能够参考精确定义的细菌分类群。临时名称如“丙酸杆菌属猫口腔分类群FOT-327”是克隆、菌株或GenBank名称或登录号可以指向的锚。未来对猫口腔细菌的下一代测序研究将能够将读段映射到分类学策划的全长16 S rRNA基因序列。
The human oral microbiome is known to play a significant role in human health and disease. While less well studied, the feline oral microbiome is thought to play a similarly important role. To determine roles oral bacteria play in health and disease, one first has to be able to accurately identify bacterial species present. 16S rRNA gene sequence information is widely used for molecular identification of bacteria and is also useful for establishing the taxonomy of novel species. The objective of this research was to obtain full 16S rRNA gene references sequences for feline oral bacteria, place the sequences in species-level phylotypes, and create a curated 16S RNA based taxonomy for common feline oral bacteria. Clone libraries were produced using “universal” and phylum-selective PCR primers and DNA from pooled subgingival plaque from healthy and periodontally diseased cats. Bacteria in subgingival samples were also cultivated to obtain isolates. Full-length 16S rDNA sequences were determined for clones and isolates that represent 171 feline oral taxa. A provisional curated taxonomy was developed based on the position of each taxon in 16S rRNA phylogenetic trees. The feline oral microbiome curated taxonomy and 16S rRNA gene reference set will allow investigators to refer to precisely defined bacterial taxa. A provisional name such as “Propionibacterium sp. feline oral taxon FOT-327” is an anchor to which clone, strain or GenBank names or accession numbers can point. Future next-generation-sequencing studies of feline oral bacteria will be able to map reads to taxonomically curated full-length 16S rRNA gene sequences.
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DOI：
10.1099/ijs.0.64169-0
发表时间：
2006-06-01
影响因子：
2.8
作者：
Yamada, Takeshi;Sekiguchi, Yuji;Kamagata, Yoichi
通讯作者：
Kamagata, Yoichi
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE
犬口腔微生物组。
影响因子：
3.7
作者：
Dewhirst FE;Klein EA;Thompson EC;Blanton JM;Chen T;Milella L;Buckley CM;Davis IJ;Bennett ML;Marshall-Jones ZV
通讯作者：
Marshall-Jones ZV
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.