Proteolytic activity of the proteasome is required for female insect reproduction.
Proteolytic activity of the proteasome is required for female insect reproduction.
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雌性昆虫繁殖需要蛋白酶体的蛋白水解活性
DOI：
10.1098/rsob.200251
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发表时间：
2021-03
期刊：
影响因子：
5.8
通讯作者：
Bao YY
中科院分区：
文献类型：
--
作者：
Wang W;Yang RR;Peng LY;Zhang L;Yao YL;Bao YY
非atp酶调控亚基（Non-ATPase regulatory subunits, Rpns）是真核生物中参与多泛素化底物识别和去泛素化的26S蛋白酶体的组成部分。本研究通过对水稻半翅目害虫褐飞虱（Nilaparvata lugens）基因组和转录组数据库的检索，鉴定出了15个Rpn及相关基因的同源序列。时空分析表明，NlRpn基因在卵子和卵巢中显著高表达，而在雄性中表达较低。RNA干扰介导的NlRpn基因缺失降低了成年女性脂肪体和卵巢中蛋白酶体的蛋白水解活性，阻碍了脂肪酶和卵黄蛋白原基因的转录，降低了卵巢中甘油三酯的含量。通过敲低NlRpns降低蛋白酶体的蛋白水解活性，还抑制了卵巢20-羟基脱皮激素（20E）生物合成途径中NlCYP307A2、NlCYP306A2和NlCYP314A1等halloween基因的转录，减少了成年雌性20E的产生，损害了卵巢发育和卵母细胞成熟，导致繁殖力下降。这些新发现表明，该蛋白酶体的蛋白水解活性是雌虫生殖过程所必需的，从而进一步加深了我们对昆虫生殖和发育策略的理解。
Non-ATPase regulatory subunits (Rpns) are components of the 26S proteasome involved in polyubiquitinated substrate recognition and deubiquitination in eukaryotes. Here, we identified 15 homologues sequences of Rpn and associated genes by searching the genome and transcriptome databases of the brown planthopper, Nilaparvata lugens, a hemipteran rice pest. Temporospatial analysis showed that NlRpn genes were significantly highly expressed in eggs and ovaries but were less-highly expressed in males. RNA interference-mediated depletion of NlRpn genes decreased the proteolytic activity of proteasome and impeded the transcription of lipase and vitellogenin genes in the fat bodies and ovaries in adult females, and reduced the triglyceride content in the ovaries. Decrease of the proteolytic activity of the proteasome via knockdown of NlRpns also inhibited the transcription of halloween genes, including NlCYP307A2, NlCYP306A2 and NlCYP314A1, in the 20-hydroxyecdysone (20E) biosynthetic pathway in the ovaries, reduced 20E production in adult females, and impaired ovarian development and oocyte maturation, resulting in reduced fecundity. These novel findings indicate that the proteolytic activity of the proteasome is required for female reproductive processes in N. lugens, thus furthering our understanding of the reproductive and developmental strategies in insects.
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影响因子：
4.6
作者：
Jiang YP;Li L;Liu ZY;You LL;Wu Y;Xu B;Ge LQ;Song QS;Wu JC
通讯作者：
Wu JC
褐飞虱 Nilaparvata lugens 先天免疫的全基因组和转录组分析
影响因子：
4.4
作者：
Bao YY;Qu LY;Zhao D;Chen LB;Jin HY;Xu LM;Cheng JA;Zhang CX
通讯作者：
Zhang CX
影响因子：
16
作者：
Ferdous, A;Gonzalez, F;Johnston, SA
通讯作者：
Johnston, SA
影响因子：
16.6
作者：
Bard JAM;Goodall EA;Greene ER;Jonsson E;Dong KC;Martin A
通讯作者：
Martin A
影响因子：
16.6
作者：
Finley D
通讯作者：
Finley D