Endocervical and vaginal microbiota in South African adolescents with asymptomatic Chlamydia trachomatis infection.
Endocervical and vaginal microbiota in South African adolescents with asymptomatic Chlamydia trachomatis infection.
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DOI：
10.1038/s41598-018-29320-x
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发表时间：
2018-07-23
影响因子：
4.6
通讯作者：
Jaspan HB
中科院分区：
文献类型：
--
作者：
Balle C;Lennard K;Dabee S;Barnabas SL;Jaumdally SZ;Gasper MA;Maseko V;Mbulawa ZZA;Williamson AL;Bekker LG;Lewis DA;Passmore JS;Jaspan HB
少女和年轻妇女是性传播感染的主要风险群体。阴道微生物群被认为在性传播感染（如沙眼衣原体）的易感性中起重要作用。我们比较了阴道侧壁和宫颈内的微生物群，并评估了与C。南非青少年的沙眼感染。通过扩增和测序16S rRNA基因的V4区和C.用分子学方法诊断沙眼。在纳入的72名女孩中，30名患有无症状C。沙眼感染三个主要的阴道群落类型被确定：一个卷曲乳杆菌，一个L。iners和一种多样的，阴道加德纳菌占主导地位。子宫颈内膜的微生物群在多样性方面与侧壁的微生物群有显著差异。在宫颈内膜和阴道侧壁之间有许多差异丰富的类群，包括西班牙无色杆菌和屎肠球菌。女人与C。沙眼衣原体G.迷走菌和其他厌氧菌。在这个非洲青少年队列中，阴道侧壁和宫颈内微生物群多样性和组成之间的显著差异是明显的，尽管两者都与C。沙眼感染
Adolescent girls and young women represent a key risk group for sexually transmitted infections (STIs). The vaginal microbiota is thought to play an important role in susceptibility to STIs such as Chlamydia trachomatis. We compared the microbiota of the lateral vaginal wall and endocervix, and assessed associations with C. trachomatis infection in South African adolescents. The endocervical and vaginal lateral wall microbiota were characterized by amplifying and sequencing the V4 region of the 16S rRNA gene and C. trachomatis diagnosed using molecular methods. Of the 72 girls included, 30 had asymptomatic C. trachomatis infections. Three major vaginal community types were identified; one Lactobacillus crispatus, one L. iners and one diverse, Gardnerella vaginalis dominant. The microbiota of the endocervix was significantly different from that of the lateral wall in terms of diversity. There were many differentially abundant taxa between the endocervix and lateral vaginal wall, including Achromobacter spanius and Enterococcus faecium. Women with C. trachomatis had higher relative abundance of G. vaginalis and other anaerobes. In this African adolescent cohort, significant differences between the lateral vaginal wall and endocervical microbiota diversity and composition were evident, although neither were strongly associated with C. trachomatis infection.
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DOI：
10.1086/657320
发表时间：
2010-12-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Brotman RM;Klebanoff MA;Nansel TR;Yu KF;Andrews WW;Zhang J;Schwebke JR
通讯作者：
Schwebke JR
影响因子：
5.7
作者：
Filardo S;Di Pietro M;Porpora MG;Recine N;Farcomeni A;Latino MA;Sessa R
通讯作者：
Sessa R
影响因子：
4.4
作者：
Fettweis JM;Serrano MG;Sheth NU;Mayer CM;Glascock AL;Brooks JP;Jefferson KK;Vaginal Microbiome Consortium (additional members);Buck GA
通讯作者：
Buck GA
影响因子：
9.4
作者：
Kim, Tae Kyung;Thomas, Susan M.;Wilson, Brenda A.
通讯作者：
Wilson, Brenda A.
影响因子：
17.1
作者：
Gajer P;Brotman RM;Bai G;Sakamoto J;Schütte UM;Zhong X;Koenig SS;Fu L;Ma ZS;Zhou X;Abdo Z;Forney LJ;Ravel J
通讯作者：
Ravel J