Retrospective Characterization of Initial Peste des petits ruminants Outbreaks (2008-2012) in the Democratic Republic of the Congo.
Retrospective Characterization of Initial Peste des petits ruminants Outbreaks (2008-2012) in the Democratic Republic of the Congo.
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DOI：
10.3390/v13122373
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发表时间：
2021-11-26
期刊：
影响因子：
--
通讯作者：
Parida S
中科院分区：
文献类型：
--
作者：
Mulumba-Mfumu LK;Mahapatra M;Diallo A;Clarke B;Twabela A;Matondo-Lusala JP;Njeumi F;Parida S
小反刍兽疫（PPR）是小反刍动物、山羊和绵羊的一种急性接触性病毒性疾病。直到2007年，刚果民主共和国（DRC）一直是一个没有PPA的国家，尽管在2006年，该国东部和西南部收到了恐慌警报，报告了反复的死亡，特别是山羊。2008年，在西部的几个村庄发现了猪肺炎疫情，导致有组织的兽医实地行动。在35个地区，酌情从临床感染和/或死亡动物中道德采集血液、拭子和病理标本，包括肺、脾、淋巴结、肾、肝和心脏组织。逐步收集了与主要风险因素和社会经济影响有关的流行病学资料，显示有744，527头山羊死亡，换算成贸易价值为35，674，600美元。金沙萨的中央兽医实验室对感染和死亡动物的样本进行了常规分析，以进行诊断，在粮农组织于2012年7月正式宣布爆发PPR疫情后，选定的组织样本被送往联合王国Pirbright研究所进行基因分型。根据2008年至2012年进行的调查，在33个检测地点中的25个（75.7%）检测到PPR病毒（PPRV）特异性抗体;在35个地点中的25个（71.4%）检测到PPRV核酸;在35个地点中的23个（65.7%）观察到PPR的典型临床表现。对2012年从刚果民主共和国Tshela收集的死亡山羊的淋巴组织中获得的PPR病毒（PPRV）的部分和全基因组序列进行分析，证实了IV系PPRV的循环，显示出最高的同源性（99.6 - 100%）与在加蓬邻国、2011年阿布米疫情和尼日利亚流行的病毒有关（99.3%同源性），尽管最近在2016年和2018年在与东非接壤的刚果民主共和国西部爆发的疫情证明了谱系II和谱系III PPRV的传播。
Peste des petits ruminants (PPR) is an acute, contagious viral disease of small ruminants, goats and sheep. The Democratic Republic of the Congo (DRC) was a PPR-free country until 2007, although in 2006, scare alerts were received from the east and the southwest of the country, reporting repeated mortalities, specifically in goats. In 2008, PPR outbreaks were seen in several villages in the west, leading to structured veterinary field operations. Blood, swabs and pathological specimens consisting of tissues from lungs, spleens, lymph nodes, kidneys, livers and hearts were ethically collected from clinically infected and/or dead animals, as appropriate, in 35 districts. Epidemiological information relating to major risk factors and socio-economic impact was progressively collected, revealing the deaths of 744,527 goats, which converted to a trade value of USD 35,674,600. Samples from infected and dead animals were routinely analyzed by the Central Veterinary Laboratory at Kinshasa for diagnosis, and after official declaration of PPR outbreaks by the FAO in July 2012, selected tissue samples were sent to The Pirbright Institute, United Kingdom, for genotyping. As a result of surveys undertaken between 2008 and 2012, PPR virus (PPRV)-specific antibodies were detected in 25 locations out of 33 tested (75.7%); PPRV nucleic acid was detected in 25 locations out of 35 (71.4%); and a typical clinical picture of PPR was observed in 23 locations out of 35 (65.7%). Analysis of the partial and full genome sequences of PPR viruses (PPRVs) obtained from lymphoid tissues of dead goats collected in Tshela in the DRC in 2012 confirmed the circulation of lineage IV PPRV, showing the highest homology (99.6−100%) with the viruses circulating in the neighboring countries of Gabon, in the Aboumi outbreak in 2011, and Nigeria (99.3% homology) in 2013, although recent outbreaks in 2016 and 2018 in the western part of the DRC that borders with East Africa demonstrated circulation of lineage II and lineage III PPRV.
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DOI：
10.3390/v13112144
发表时间：
2021-10-25
期刊：
Viruses
影响因子：
--
作者：
Mahapatra M;Pachauri R;Subramaniam S;Banyard AC;ChandraSekar S;Ramakrishnan MA;Njeumi F;Muthuchelvan D;Parida S
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Parida S
DOI：
10.1128/genomea.01023-14
发表时间：
2014-10-23
期刊：
Genome announcements
影响因子：
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作者：
Muniraju M;Munir M;Banyard AC;Ayebazibwe C;Wensman J;Zohari S;Berg M;Parthiban AR;Mahapatra M;Libeau G;Batten C;Parida S
通讯作者：
Parida S
DOI：
10.3390/v6062287
发表时间：
2014-06-06
期刊：
Viruses
影响因子：
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作者：
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通讯作者：
Ly H
影响因子：
2.7
作者：
Mantip, Samuel;Quan, Melvyn;van Vuuren, Moritz
通讯作者：
van Vuuren, Moritz
影响因子：
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作者：
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通讯作者：
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