Intracellular Propionibacterium acnes infection in glandular epithelium and stromal macrophages of the prostate with or without cancer.
Intracellular Propionibacterium acnes infection in glandular epithelium and stromal macrophages of the prostate with or without cancer.
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DOI：
10.1371/journal.pone.0090324
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Eishi Y
中科院分区：
文献类型：
--
作者：
Bae Y;Ito T;Iida T;Uchida K;Sekine M;Nakajima Y;Kumagai J;Yokoyama T;Kawachi H;Akashi T;Eishi Y
最近关于痤疮丙酸杆菌（P.acnes）的报告表明，这种细菌在前列腺中普遍存在，与急性和慢性前列腺炎症有关，并且可能在前列腺癌发生中发挥作用。为了评估这种本土细菌的致病作用，我们使用酶免疫组织化学法，使用新型痤疮丙酸杆菌特异性单克隆抗体（PAL 抗体）和抗核因子-κ B (NF-κB) 抗体，筛选了根治性前列腺切除术标本中的细菌。我们检查了 28 名前列腺癌患者和 18 名年龄匹配的膀胱癌对照患者的根治性前列腺切除术标本的福尔马林固定和石蜡包埋的组织切片，但没有前列腺癌。 PAL 抗体的免疫组织化学显示，在大多数前列腺样本中，一些非癌性腺上皮和基质巨噬细胞内存在小圆形体。与对照样本相比，前列腺癌样本的细胞质痤疮丙酸杆菌或腺上皮细胞核 NF-κB 表达的频率较高，并且基质巨噬细胞的痤疮丙酸杆菌数量较多。这些参数在前列腺的外周区也高于移行区，特别是在前列腺癌样本中。核 NF-κB 表达在有痤疮丙酸杆菌的腺体中比在没有痤疮丙酸杆菌的腺体中更频繁。带有细菌的基质巨噬细胞的数量与 PZ 和 TZ 区域的慢性炎症等级以及 TZ 区域的急性炎症等级相关。使用新型单克隆抗体检测前列腺中的痤疮丙酸杆菌进行的免疫组织化学分析表明，非癌性前列腺中的上皮内痤疮丙酸杆菌感染以及该细菌引起的炎症可能导致前列腺癌的发生。
Recent reports on Propionibacterium acnes (P. acnes) suggest that this bacterium is prevalent in the prostate, is associated with acute and chronic prostatic inflammation, and might have a role in prostate carcinogenesis. To evaluate the pathogenic role of this indigenous bacterium, we screened for the bacterium in radical prostatectomy specimens using enzyme immunohistochemistry with a novel P. acnes-specific monoclonal antibody (PAL antibody), together with an anti-nuclear factor-kappa B (NF-κB) antibody. We examined formalin-fixed and paraffin-embedded tissue sections of radical prostatectomy specimens from 28 patients with prostate cancer and 18 age-matched control patients with bladder cancer, but without prostate cancer. Immunohistochemistry with the PAL antibody revealed small round bodies within some non-cancerous glandular epithelium and stromal macrophages in most prostate samples. Prostate cancer samples had higher frequencies of either cytoplasmic P. acnes or nuclear NF-κB expression of glandular epithelium and higher numbers of stromal macrophages with P. acnes than control samples. These parameters were also higher in the peripheral zone than in the transitional zone of the prostate, especially in prostate cancer samples. Nuclear NF-κB expression was more frequent in glands with P. acnes than in glands without P. acnes. The number of stromal macrophages with the bacterium correlated with the grade of chronic inflammation in both the PZ and TZ areas and with the grade of acute inflammation in the TZ area. Immunohistochemical analysis with a novel monoclonal antibody for detecting P. acnes in the prostate suggested that intraepithelial P. acnes infection in non-cancerous prostate glands and inflammation caused by the bacterium may contribute to the development of prostate cancer.
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DOI：
10.1038/modpathol.2012.80
发表时间：
2012-09
期刊：
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
影响因子：
--
作者：
通讯作者：
--
影响因子：
168.9
作者：
Grönberg, H
通讯作者：
Grönberg, H
影响因子：
6.6
作者：
Lee, JC;Muller, CH;Berger, RE
通讯作者：
Berger, RE
影响因子：
3.8
作者：
Furukawa, Asuka;Uchida, Keisuke;Eishi, Yoshinobu
通讯作者：
Eishi, Yoshinobu
影响因子：
6.6
作者：
Berger, RE;Krieger, JN;Hillier, SL
通讯作者：
Hillier, SL