Adenovirus associated with acute diarrhea: a case-control study.
Adenovirus associated with acute diarrhea: a case-control study.
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DOI：
10.1186/s12879-018-3340-1
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发表时间：
2018-09-03
影响因子：
3.7
通讯作者：
Ma XJ
中科院分区：
文献类型：
--
作者：
Qiu FZ;Shen XX;Li GX;Zhao L;Chen C;Duan SX;Guo JY;Zhao MC;Yan TF;Qi JJ;Wang L;Feng ZS;Ma XJ
腹泻是低收入和中等收入国家幼儿发病率和死亡率的主要来源。人腺病毒（HAdV），特别是HAdV F种（40，41）已被认为是重要的致病病原体，然而，与急性胃肠炎相关的其他HAdV的分子流行病学数据有限。在本研究中，我们进行了一项病例对照研究，包括273名儿童出现腹泻病和361名健康儿童匹配的对照，在河北省儿童医院（中国），以探讨非肠道HAdV与腹泻的关系。采用实时定量PCR（qPCR）检测和定量HAdV，并通过测序和系统发育分析进行血清分型。采用比值比（OR）分析HAdV感染的危险因素。HAdV检出率为79 273例腹泻患儿中有7种血清型，占28.94 HAdV 40、41、3、2、1、5和57型，其中血清型40、41和3占优势，26 361名健康儿童中，HAdV40、41、3、2、1、5、57、6和31型阳性率为7.20%。HAdV阳性率在腹泻儿童中占91.14%，在< 3岁的对照组中占65.38%。病毒载量在病例组和对照组之间以及Ad41阳性患者和健康对照组之间均无显著差异。除HAdV40和41外，HAdV3也与腹泻有关（OR = 17.301，校正OR = 9.205，p < 0.001）。我们的研究结果表明，在腹泻和健康儿童中存在高度多样性的HAdV，并暗示非肠道HAdV3可能导致腹泻。
Diarrhea is a major source of morbidity and mortality among young children in low-income and middle-income countries. Human adenoviruses (HAdV), particular HAdV species F (40, 41) has been recognized as important causal pathogens, however limited data exist on molecular epidemiology of other HAdV associated with acute gastroenteritis. In the present preliminary study, we performed a case-control study involving 273 children who presented diarrheal disease and 361 healthy children matched control in Children’s hospital of Hebei Province (China) to investigate the relationship between non-enteric HAdV and diarrhea. HAdV were detected and quantified using quantitative real-time PCR (qPCR) and serotyped by sequencing and phylogenetic analysis. Odds ratio (OR) was used to assess the risk factor of HAdV. HAdV were detected in 79 (28.94%) of 273 children with diarrhea including 7 different serotypes (HAdV 40, 41, 3, 2,1,5 and 57) with serotypes 40, 41 and 3 being the most dominant and in 26 (7.20%) of 361 healthy children containing 9 serotypes (HAdV 40, 41, 3, 2,1,5,57,6 and 31). A majority (91.14%) of HAdV positives occurred in diarrhea children and 65.38% in controls< 3 years of age. No significant difference in the viral load was found between case and control groups or between Ad41-positive patients and healthy controls. In addition to HAdV 40 and 41, HAdV 3 was also associated with diarrhea (OR = 17.301, adjusted OR = 9.205, p < 0.001). Our results demonstrate a high diversity of HAdV present among diarrhea and healthy children and implicate that non-enteric HAdV3 may lead to diarrhea.
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影响因子：
3.7
作者：
Liu L;Qian Y;Zhang Y;Deng J;Jia L;Dong H
通讯作者：
Dong H
DOI：
10.1016/j.cmi.2016.11.004
发表时间：
2017-03
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
Lin MR;Yang SL;Gong YN;Kuo CC;Chiu CH;Chen CJ;Hsieh YC;Kuo CY;Fang CW;Tsao KC;Huang YC
通讯作者：
Huang YC
影响因子：
12.7
作者：
Afrad, Mokibul Hassan;Avzun, Tasnuva;Rahman, Mustafizur
通讯作者：
Rahman, Mustafizur
影响因子：
9.4
作者：
Dey, Rakhi Sharma;Ghosh, Santanu;Ramamurthy, Thandavarayan
通讯作者：
Ramamurthy, Thandavarayan
影响因子：
2.3
作者：
Colak, Meryem;Bozdayi, Gulendam;Ozkan, Secil
通讯作者：
Ozkan, Secil