Comparison of novel MLB-clade, VA-clade and classic human astroviruses highlights constrained evolution of the classic human astrovirus nonstructural genes.
Comparison of novel MLB-clade, VA-clade and classic human astroviruses highlights constrained evolution of the classic human astrovirus nonstructural genes.
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DOI：
10.1016/j.virol.2012.09.040
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发表时间：
2013-02-05
期刊：
影响因子：
3.7
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Jiang H;Holtz LR;Bauer I;Franz CJ;Zhao G;Bodhidatta L;Shrestha SK;Kang G;Wang D
八种血清型的人类星状病毒（经典的人类星状病毒）是引起腹泻的病原体。最近，在人类粪便中发现了另外五种属于两个不同分支的星状病毒，包括星状病毒MLB1、MLB2、VA1、VA2和VA3。我们报道在人类粪便中发现了两种新的星状病毒，星状病毒MLB3和VA4。对这两种病毒和先前描述的星状病毒VA2和VA3进行全基因组测序，获得MLB和VA分支的7个完整基因组，用于与经典人类星状病毒进行比较分析。比较经典人类、MLB和VA进化支的蛋白酶、聚合酶和衣壳的遗传距离、同义位点的同义突变数（dS）、非同义位点的非同义突变数（dN）和dN/dS比值，表明经典人类星状病毒的蛋白酶和聚合酶承受着不同的选择压力。
Eight serotypes of human astroviruses (the classic human astroviruses) are causative agents of diarrhea. Recently, five additional astroviruses belonging to two distinct clades have been described in human stool, including astroviruses MLB1, MLB2, VA1, VA2 and VA3. We report the discovery in human stool of two novel astroviruses, astroviruses MLB3 and VA4. The complete genomes of these two viruses and the previously described astroviruses VA2 and VA3 were sequenced, affording 7 complete genomes from the MLB and VA clades for comparative analysis to the classic human astroviruses. Comparison of the genetic distance, number of synonymous mutations per synonymous site (dS), number of non-synonymous mutations per non-synonymous site (dN) and the dN/dS ratio in the protease, polymerase and capsid of the classic human, MLB and VA clades suggests that the protease and polymerase of the classic human astroviruses are under distinct selective pressure.
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影响因子：
4.8
作者：
Finkbeiner SR;Holtz LR;Jiang Y;Rajendran P;Franz CJ;Zhao G;Kang G;Wang D
通讯作者：
Wang D
影响因子：
2.7
作者：
Walter JE;Briggs J;Guerrero ML;Matson DO;Pickering LK;Ruiz-Palacios G;Berke T;Mitchell DK
通讯作者：
Mitchell DK
人腹泻的宏基因组分析：病毒检测和发现。
影响因子：
6.7
作者：
Finkbeiner, Stacy R.;Allred, Adam F.;Tarr, Phillip I.;Klein, Eileen J.;Kirkwood, Carl D.;Wang, David
通讯作者：
Wang, David
影响因子：
3.1
作者：
KJELDSBERG, E
通讯作者：
KJELDSBERG, E
影响因子：
5.4
作者：
Finkbeiner, Stacy R.;Li, Yan;Tong, Suxiang
通讯作者：
Tong, Suxiang