A molecular epidemiological study of respiratory viruses detected in Japanese children with acute wheezing illness.
A molecular epidemiological study of respiratory viruses detected in Japanese children with acute wheezing illness.
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DOI：
10.1186/1471-2334-11-168
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发表时间：
2011-06-10
影响因子：
3.7
通讯作者：
Kimura H
中科院分区：
文献类型：
--
作者：
Fujitsuka A;Tsukagoshi H;Arakawa M;Goto-Sugai K;Ryo A;Okayama Y;Mizuta K;Nishina A;Yoshizumi M;Kaburagi Y;Noda M;Tashiro M;Okabe N;Mori M;Yokota S;Kimura H
最近的研究强烈表明，一些呼吸道病毒与急性喘息和/或支气管哮喘加重的诱导有关。然而，这些病毒的分子流行病学尚不完全清楚。利用聚合酶链反应（PCR）技术，对115名日本儿童进行了多种呼吸道病毒检测。此外，检测到的病毒进行同源性、配对距离和系统发育分析。99例（86.1%）患者检出病毒。单独检测呼吸道合胞病毒（RSV） 47例（40.9%），单独检测人鼻病毒（HRV） 36例（31.3%）。14例（12.2%）患者同时检测到RSV和HRV。单独检出人偏肺病毒（HMPV）和单独检出人副流感病毒（HPIV）各1例（0.9%）。同源性和系统发育分析表明，RSV和HRV毒株在遗传上属于不同的种或亚群。此外，在没有喘息病史的患者中检测到的优势病毒是RSV，而在有喘息病史的患者中检测到的优势病毒是HRV。结果表明，这些遗传多样性的呼吸道病毒，特别是RSV和HRV，可能与日本儿童的喘息有关。
Recent studies strongly suggest that some respiratory viruses are associated with the induction of acute wheezing and/or exacerbation of bronchial asthma. However, molecular epidemiology of these viruses is not exactly known. Using PCR technology, we attempted to detect various respiratory viruses from 115 Japanese children. Furthermore, the detected viruses were subjected to homology, pairwise distance, and phylogenetic analysis. Viruses were detected from 99 (86.1%) patients. Respiratory syncytial virus (RSV) alone and human rhinovirus (HRV) alone were detected in 47 (40.9%) and 36 (31.3%) patients, respectively. Both RSV and HRV were detected in 14 (12.2%) patients. Human metapneumovirus (HMPV) alone and human parainfluenza virus (HPIV) alone were detected in 1 (0.9%) patient each, respectively. Homology and phylogenetic analyses showed that the RSV and HRV strains were classified into genetically diverse species or subgroups. In addition, RSV was the dominant virus detected in patients with no history of wheezing, whereas HRV was dominant in patients with a history of wheezing. The results suggested that these genetically diverse respiratory viruses, especially RSV and HRV, might be associated with wheezing in Japanese children.
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DOI：
10.1016/j.jaci.2008.10.007
发表时间：
2009-01
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Miller EK;Edwards KM;Weinberg GA;Iwane MK;Griffin MR;Hall CB;Zhu Y;Szilagyi PG;Morin LL;Heil LH;Lu X;Williams JV;New Vaccine Surveillance Network
通讯作者：
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作者：
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DOI：
10.1073/pnas.0504666102
发表时间：
2005-09-06
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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