Genetic characterization of specific pathogen-free rhesus macaque (Macaca mulatta) populations at the California National Primate Research Center (CNPRC).
Genetic characterization of specific pathogen-free rhesus macaque (Macaca mulatta) populations at the California National Primate Research Center (CNPRC).
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DOI：
10.1002/ajp.20811
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发表时间：
2010-06
影响因子：
2.4
通讯作者：
Smith, David Glenn
中科院分区：
文献类型：
--
作者：
Kanthaswamy, Sree;Kou, Alex;Satkoski, Jessica;Penedo, Maria Cecilia T.;Ward, Thea;Ng, Jillian;Gill, Leanne;Lerche, Nicholas W.;Erickson, Bethany J-A;Smith, David Glenn
关键词：
一项基于14个STR的研究旨在了解CNPRC常规SPF(无特定病原体)和超SPF圈养猕猴种群相对于其传统创建者发生的代际遗传变化。代际遗传漂移导致每个研究群体的年龄队列，特别是传统群体中的年龄队列，彼此之间以及与其创始人之间的差异越来越大。虽然传统种群和两个SPF种群中的任何一个之间仍然只有最小的分层，但常规SPF和超SPF动物是从它们的传统创始人中分离出来的，这导致这两个SPF种群保持着略微不同的彼此。常规SPF群体，特别是超SPF群体，受到不同祖先、采样和稀有等位基因丢失的影响，导致这些亚群之间存在很大程度的遗传分化。创始人的原籍国是中国石油天然气集团公司SPF股票MHC单倍型组成的主要决定因素。选择携带所需MAMU-A*01或-B*01基因的SPF群体育种者并没有影响常规群体和SPF研究群体的总体遗传异质性。由于错误地对研究种群的祖先进行分类可能会通过排除具有地区特定基因类型或重要表型的动物而破坏实验结果，因此了解创始人/后代的遗传关系对于研究具有不同地理起源的候选基因至关重要。结合人口统计管理，SPF群体的群体遗传评估可以减少研究种群之间的过度表型差异，并促进成功的生产目标。
A study based on 14 STRs was conducted to understand intergenerational genetic changes that have occurred within the CNPRC’s regular SPF (Specific pathogen-free) and super-SPF captive rhesus macaque populations relative to their conventional founders. Intergenerational genetic drift has caused age cohorts of each study population, especially within the conventional population, to become increasingly differentiated from each other and from their founders. While there is still only minimal stratification between the conventional population and either of the two SPF populations, the separate derivation of the regular SPF and super-SPF animals from their conventional founders has caused the two SPF populations to remain marginally different from each other. The regular SPF and, especially, the super-SPF populations have been influenced by the effects of differential ancestry, sampling and lost rare alleles, causing a substantial degree of genetic divergence between these subpopulations. The country of origin of founders is the principal determinant of the MHC haplotype composition of the SPF stocks at the CNPRC. Selection of SPF colony breeders bearing desired genotypes of Mamu-A*01 or –B*01 has not affected the overall genetic heterogeneity of the conventional and the SPF research stocks. Because misclassifying the ancestry of research stocks can undermine experimental outcomes by excluding animals with regional-specific genotypes or phenotypes of importance, understanding founder/descendent genetic relationships is crucial for investigating candidate genes with distinct geographic origins. Together with demographic management, population genetic assessments of SPF colonies can curtail excessive phenotypic variation among the study stocks and facilitate successful production goals.
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影响因子：
3.8
作者：
Huff, JL;Eberle, R;Barry, PA
通讯作者：
Barry, PA
影响因子：
1.6
作者：
Kanthaswamy, Sree;Tom, Bradley K.;Koskinen, Mikko T.
通讯作者：
Koskinen, Mikko T.
影响因子：
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作者：
Knapp, LA;Lehmann, E;Watkins, DI
通讯作者：
Watkins, DI
影响因子：
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作者：
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通讯作者：
Cabello, PH
影响因子：
2.4
作者：
Kanthaswamy, S;Von Dollen, A;Smith, DG
通讯作者：
Smith, DG