Circular RNA hsa_circ_0000745 may serve as a diagnostic marker for gastric cancer.
Circular RNA hsa_circ_0000745 may serve as a diagnostic marker for gastric cancer.
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环状RNA hsa_circ_0000745可作为胃癌的诊断标志物
DOI：
10.3748/wjg.v23.i34.6330
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发表时间：
2017-09-14
影响因子：
4.3
通讯作者：
Zhu ZQ
中科院分区：
文献类型：
--
作者：
Huang M;He YR;Liang LC;Huang Q;Zhu ZQ
确定环状rna （circRNAs）是否参与胃癌（GC）的病理过程。随机选取3个在胃癌和结直肠癌中有差异表达的circrna，采用定量逆转录聚合酶链反应（qRT-PCR）方法对20对胃组织和正常组织进行验证。基于预测的circRNA-miRNA网络，我们将重点放在hsa_circ_0000745上，发现与正常组织相比，hsa_circ_0000745在20个GC组织中下调。通过qRT-PCR进一步分析60例胃癌组织和配对的邻近非肿瘤组织、60例胃癌患者血浆样本和60例健康对照血浆样本的hsa_circ_0000745水平。统计评估hsa_circ_0000745水平与GC患者临床病理特征之间的关系。采用受试者工作特征（ROC）曲线评价hsa_circ_0000745在GC中的诊断价值。Hsa_circ_0000745在胃癌组织中与非肿瘤组织相比下调（P < 0.001），在胃癌患者的血浆样本中与健康对照相比下调（P < 0.001）。hsa_circ_0000745在胃癌组织中的表达水平与肿瘤分化相关，而在血浆中的表达水平与肿瘤-淋巴结-转移分期相关。血浆中hsa_circ_0000745的ROC曲线下面积（AUC）为0.683，具有较好的诊断价值。血浆hsa_circ_0000745水平联合癌胚性抗原（CEA）水平使AUC升高至0.775。Hsa_circ_0000745在胃癌中发挥着重要作用，其在血浆中的表达水平与CEA水平结合，是一种有前景的胃癌诊断标志物。
To determine whether circular RNAs (circRNAs) are involved in pathological processes of gastric cancer (GC). Three circRNAs with differential expression in GC and colorectal cancer were randomly selected for validation by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), using 20 pairs of gastric tissues and normal tissues. Based on the predicted circRNA-miRNA network, we then focused on hsa_circ_0000745, which was found to be down-regulated in 20 GC tissues compared with normal tissues. The hsa_circ_0000745 levels were further analyzed by qRT-PCR in 60 GC tissues and paired adjacent non-tumor tissues, as well as 60 plasma samples from GC patients and 60 plasma samples from healthy controls. The associations between the levels of hsa_circ_0000745 and the clinicopathological features of GC patients were statistically assessed. A receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of hsa_circ_0000745 in GC. Hsa_circ_0000745 was down-regulated in GC tissues vs non-tumorous tissues (P < 0.001) and in plasma samples from patients with GC vs healthy controls (P < 0.001). The expression level of hsa_circ_0000745 in GC tissues correlated with tumor differentiation, while the expression level in plasma correlated with tumor-node-metastasis stage. The area under the ROC curve (AUC) of hsa_circ_0000745 in plasma was 0.683, suggesting good diagnostic value. Plasma hsa_circ_0000745 level combined with carcinoembryogenic antigen (CEA) level increased the AUC to 0.775. Hsa_circ_0000745 plays an important role in GC and its expression level in plasma in combination with CEA level is a promising diagnostic marker for this malignancy.
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影响因子：
--
作者：
Li F;Zhang L;Li W;Deng J;Zheng J;An M;Lu J;Zhou Y
通讯作者：
Zhou Y
影响因子：
4.6
作者：
Bachmayr-Heyda A;Reiner AT;Auer K;Sukhbaatar N;Aust S;Bachleitner-Hofmann T;Mesteri I;Grunt TW;Zeillinger R;Pils D
通讯作者：
Pils D
circRNA生物合成的调控
影响因子：
4.1
作者：
Chen, Ling-Ling;Yang, Li
通讯作者：
Yang, Li
影响因子：
5.8
作者：
Cheng, Jun;Metge, Franziska;Dieterich, Christoph
通讯作者：
Dieterich, Christoph
影响因子：
254.7
作者：
Torre, Lindsey A.;Bray, Freddie;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin