Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538.
Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538.
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DOI：
10.1371/journal.pone.0164815
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Luo P
中科院分区：
文献类型：
--
作者：
Zhong S;Ma L;Fatima SA;Yang J;Chen W;Liu T;Hu Y;Li Q;Guo J;Zhang M;Lei L;Li X;Tang S;Luo P
小麦白粉病抗性基因Pm40位于7BS染色体臂上，对几乎所有流行的Blumeria graminis f小种均有效。小麦白粉病（Bgt）是我国常见的小麦种质PI 672538携带的小麦病原菌。利用抗性PI 672538与感病品系L1034杂交得到的一组F1、F2和F2:3群体，进行白粉病抗性遗传分析，并构建了Pm40基因的高密度连锁图谱。我们构建了总长度为 6.18 cM、标记之间平均间距为 0.48 cM 的高密度连锁遗传图谱。在删除箱 C-7BS-1-0.27 中，Pm40 两侧是 Xwmc335 和 BF291338，遗传距离分别为 0.58 cM 和 0.26 cM。基于EST-STS标记的比较基因组分析建立了Pm40基因组区域与短柄草染色体3上的1.09-Mbp基因组区域、水稻8号染色体上的1.16-Mbp基因组区域和高粱7号染色体上的1.62-Mbp基因组区域的高度共线性。我们进一步将Pm40目标区间锚定到小麦基因组序列。构建了 7BS 上包含 97 个基因的 85 个小麦重叠群的假定线性索引。总共有 9 个基因可以被认为是目标基因组区域中白粉病抗性的候选基因，这些基因编码参与植物防御和对病原体攻击的反应的蛋白质。这些结果将有助于开发新的标记，用于小麦育种计划中 Pm40 的图位克隆和标记辅助选择。
The wheat powdery mildew resistance gene Pm40, which is located on chromosomal arm 7BS, is effective against nearly all prevalent races of Blumeria graminis f. sp tritici (Bgt) in China and is carried by the common wheat germplasm PI 672538. A set of the F1, F2 and F2:3 populations from the cross of the resistant PI 672538 with the susceptible line L1034 were used to conduct genetic analysis of powdery mildew resistance and construct a high-density linkage map of the Pm40 gene. We constructed a high-density linkage genetic map with a total length of 6.18 cM and average spacing between markers of 0.48 cM.Pm40 is flanked by Xwmc335 and BF291338 at genetic distances of 0.58 cM and 0.26 cM, respectively, in deletion bin C-7BS-1-0.27. Comparative genomic analysis based on EST-STS markers established a high level of collinearity of the Pm40 genomic region with a 1.09-Mbp genomic region on Brachypodium chromosome 3, a 1.16-Mbp genomic region on rice chromosome 8, and a 1.62-Mbp genomic region on sorghum chromosome 7. We further anchored the Pm40 target intervals to the wheat genome sequence. A putative linear index of 85 wheat contigs containing 97 genes on 7BS was constructed. In total, 9 genes could be considered as candidates for the resistances to powdery mildew in the target genomic regions, which encoded proteins that were involved in the plant defense and response to pathogen attack. These results will facilitate the development of new markers for map-based cloning and marker-assisted selection of Pm40 in wheat breeding programs.
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DOI：
10.1073/pnas.1016981108
发表时间：
2011-05-10
影响因子：
11.1
作者：
Cao, Aizhong;Xing, Liping;Chen, Peidu
通讯作者：
Chen, Peidu
影响因子：
56.9
作者：
Krattinger, Simon G.;Lagudah, Evans S.;Keller, Beat
通讯作者：
Keller, Beat
影响因子：
2.7
作者：
Huang, XQ;Hsam, SLK;Zeller, FJ
通讯作者：
Zeller, FJ
DOI：
10.1073/pnas.2435133100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Feuillet, C;Travella, S;Keller, B
通讯作者：
Keller, B
影响因子：
5.4
作者：
Huang, X. Q.;Coester, H.;Roeder, M. S.
通讯作者：
Roeder, M. S.