Stem cells with neural crest characteristics derived from the bulge region of cultured human hair follicles.
Stem cells with neural crest characteristics derived from the bulge region of cultured human hair follicles.
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DOI：
10.1038/jid.2009.322
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发表时间：
2010-05
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影响因子：
--
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在这项研究中，我们证明，我们可以分离的干细胞（SC）与神经嵴的特点，从隆突区培养的人毛囊（HF）。这些干细胞可原位增殖，形成球状结构，附着于成纤维细胞的隆突区，表达未成熟的神经嵴细胞标记，但不表达分化标记。一项表达谱研究表明，它们与小鼠皮肤未成熟神经嵴细胞具有相似的基因表达模式。这些人SC是标记保留细胞，并且能够通过体外不对称细胞分裂进行自我更新。它们表现出克隆多能性，在体外克隆单细胞培养后可以产生肌源性、黑素细胞和神经元细胞谱系。此外，这些SC显示出向间充质谱系分化的潜力，并且它们可以分化成脂肪细胞、软骨细胞和骨细胞谱系。这些细胞的神经元分化诱导全局基因表达的变化与神经元相关基因的表达显着增加。分化的神经元细胞可以在小鼠脑中持续存在并保留神经元分化标记物。具有神经嵴特征的干细胞在高频中的存在可能为这些细胞在再生医学中的应用提供新的机会。
In this study, we demonstrate that we can isolate stem cells (SCs) with neural crest characteristics from the bulge area of cultured human hair follicles (HFs). These SCs can proliferate in situ and form spheroid structures attached to the bulge area of HFs, and they express immature neural crest cell markers but not differentiation markers. An expression profiling study showed that they share a similar gene expression pattern with murine skin immature neural crest cells. These human SCs are label-retaining cells and are capable of self-renewal through asymmetric cell division in vitro. They exhibit clonal multipotency that can give rise to myogenic, melanocytic, and neuronal cell lineages after in vitro clonal single cell culture. In addition, these SCs show differentiation potential toward mesenchymal lineages, and they can be differentiated into adipocyte, chondrocyte, and osteocyte lineages. Neuronal differentiation of these cells induces global gene expression changes with a significantly increased expression of neuron-associated genes. Differentiated neuronal cells can persist in mouse brain and retain neuronal differentiation markers. The presence of SCs with neural crest characteristics in HFs may offer new opportunities for the use of these cells in regenerative medicine.
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影响因子：
46.9
作者：
Morris, RJ;Liu, YP;Cotsarelis, G
通讯作者：
Cotsarelis, G
影响因子：
5.2
作者：
Hu, Yao Fei;Zhang, Zhi-Jian;Sieber-Blum, Maya
通讯作者：
Sieber-Blum, Maya
DOI：
10.1073/pnas.0501263102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Amoh, Y;Li, L;Hoffman, RM
通讯作者：
Hoffman, RM
影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD
影响因子：
2.5
作者：
Sieber-Blum, M;Grim, M;Szeder, V
通讯作者：
Szeder, V