Clonal behaviour of myogenic precursor cells throughout the vertebrate lifespan.
Clonal behaviour of myogenic precursor cells throughout the vertebrate lifespan.
复制标题
DOI：
10.1242/bio.059476
复制
发表时间：
2022-08-15
期刊：
影响因子：
2.4
通讯作者：
Xu, Qiling
中科院分区：
文献类型：
--
作者：
Hughes, Simon M.;Escaleira, Roberta C.;Wanders, Kees;Koth, Jana;Wilkinson, David G.;Xu, Qiling
为了解决骨骼肌发生过程中干细胞多样性的问题，通过在斑马鱼中捕获增强子，获得了一种类似brainbow的遗传细胞谱系追踪方法，称为Musclebow2。结果表明，在原发性肌瘤形成后，每个小体至少有15个肌肉前体细胞（mpcs），它们在那里增殖，但在孵化前对肌肉生长贡献不大。此后，真皮细胞衍生的mpc克隆迅速扩增，而一些后代则经历终末分化，导致mpc池内随机克隆漂移。未获得基于细胞系的克隆命运多样性的证据。在未受伤的动物中未观察到纤维和mpc死亡。单个有标记的肌纤维在生命的大部分时间内持续存在，表明核更新率较低。在成年期，早期标记的mpc克隆标记稳定的组织块，包括相当一部分外轴体或下轴体。来自不同的早期标记克隆的细胞融合发生在克隆重叠区域。伤口从几个局部mpcs再生；没有获得专门的干细胞MPCS的证据。总之，我们的数据表明，肌肉组织中的大多数mpcs有助于局部生长和修复，并且表明在没有创伤的情况下，细胞更新很低。摘要：引入Musclebow2克隆细胞系分析，揭示斑马鱼整个生命周期骨骼肌形成、修复和维持的细胞动力学。
To address questions of stem cell diversity during skeletal myogenesis, a Brainbow-like genetic cell lineage tracing method, dubbed Musclebow2, was derived by enhancer trapping in zebrafish. It is shown that, after initial formation of the primary myotome, at least 15 muscle precursor cells (mpcs) seed each somite, where they proliferate but contribute little to muscle growth prior to hatching. Thereafter, dermomyotome-derived mpc clones rapidly expand while some progeny undergo terminal differentiation, leading to stochastic clonal drift within the mpc pool. No evidence of cell-lineage-based clonal fate diversity was obtained. Neither fibre nor mpc death was observed in uninjured animals. Individual marked muscle fibres persist across much of the lifespan indicating low rates of nuclear turnover. In adulthood, early-marked mpc clones label stable blocks of tissue comprising a significant fraction of either epaxial or hypaxial somite. Fusion of cells from separate early-marked clones occurs in regions of clone overlap. Wounds are regenerated from several local mpcs; no evidence for specialised stem mpcs was obtained. In conclusion, our data indicate that most mpcs in muscle tissue contribute to local growth and repair and suggest that cellular turnover is low in the absence of trauma. Summary: Musclebow2 clonal cell lineage analysis is introduced to reveal the cellular dynamics of skeletal muscle formation, repair and maintenance throughout the life of zebrafish.
登录
查看更多内容
影响因子：
7.7
作者：
Ganassi M;Badodi S;Wanders K;Zammit PS;Hughes SM
通讯作者：
Hughes SM
影响因子：
3.7
作者：
DIMARIO, JX;STOCKDALE, FE
通讯作者：
STOCKDALE, FE
DOI：
10.1073/pnas.2012450117
发表时间：
2020-12-08
影响因子：
11.1
作者：
Kelu JJ;Pipalia TG;Hughes SM
通讯作者：
Hughes SM
影响因子：
10.5
作者：
Kassar-Duchossoy, L;Giacone, E;Tajbakhsh, S
通讯作者：
Tajbakhsh, S
影响因子：
56.9
作者：
Gurevich, David B.;Phong Dang Nguyen;Currie, Peter D.
通讯作者：
Currie, Peter D.