Highly sensitive detection of HER2 extracellular domain in the serum of breast cancer patients by piezoelectric microcantilevers.
Highly sensitive detection of HER2 extracellular domain in the serum of breast cancer patients by piezoelectric microcantilevers.
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DOI：
10.1021/ac103301r
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发表时间：
2011-05-01
影响因子：
7.4
通讯作者：
Adams, Gregory P.
中科院分区：
文献类型：
--
作者：
Loo, LiNa;Capobianco, Joseph A.;Wu, Wei;Gao, Xiaotong;Shih, Wan Y.;Shih, Wei-Heng;Pourrezaei, Kambiz;Robinson, Matthew K.;Adams, Gregory P.
快速、灵敏地检测血清肿瘤生物标志物是监测癌症患者疾病进展的必要条件。高灵敏度压电微悬臂传感器（PEMS）为生物标志物检测提供了一种有吸引力的工具，但其在血清中遇到的复杂环境中的实用性尚未确定。作为概念的证明，我们已经用特异性结合HER2的抗体功能化了PEMS， HER2是一种生物标志物（抗原），在乳腺癌患者的血液中通常过表达。这些抗HER2 PEMS生物传感器的功能和敏感性是通过将重组HER2加入人血清中进行初步评估的。然后确定它们检测乳腺癌患者血清中存在的天然HER2的能力。我们发现抗HER2 PEMS能够准确地检测重组HER2和临床相关水平（bbb20 ng/ml）的自然发生的HER2。这表明基于pems的生物传感器为生物标志物检测提供了一种潜在的有效工具。
Rapid and sensitive detection of serum tumor biomarkers are needed to monitor cancer patients for disease progression. Highly sensitive piezoelectric microcantilever sensors (PEMS) offer an attractive tool for biomarker detection, however their utility in the complex environment encountered in serum has yet to be determined. As a proof of concept, we have functionalized PEMS with antibodies that specifically bind to HER2, a biomarker (antigen) that is commonly overexpressed in the blood of breast cancer patients. The function and sensitivity of these anti-HER2 PEMS biosensors was initially assessed using recombinant HER2 spiked into human serum. Their ability to detect native HER2 present in the serum of breast cancer patients was then determined. We have found that the anti-HER2 PEMS were able to accurately detect both recombinant and naturally occurring HER2 at clinically relevant levels (>2 ng/ml). This indicates that PEMS-based biosensors provide a potentially effective tool for biomarker detection.
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