The Chronic Wound Phageome: Phage Diversity and Associations with Wounds and Healing Outcomes.
The Chronic Wound Phageome: Phage Diversity and Associations with Wounds and Healing Outcomes.
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慢性创伤噬菌体组：噬菌体多样性与创伤和愈合结果的关联。
DOI：
10.1128/spectrum.02777-21
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发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
Chen, Irene A.
中科院分区：
文献类型：
--
作者：
Verbanic, Samuel;Deacon, John M.;Chen, Irene A.
慢性伤口愈合的两个主要障碍是多种微生物感染和生物膜形成。最近的研究已经表征了这些微生物群的细菌部分，并且已经开始阐明与愈合结果的组成相关性。然而，推动成分变化的因素仍在探索中。病毒组可能在塑造细菌群落结构和功能方面发挥重要作用。先前对皮肤病毒组的研究确定，它是由噬菌体，感染细菌的病毒主导的。为了表征病毒组，我们招募了20名在门诊伤口护理诊所就诊的慢性伤口患者进行微生物组调查，在单次锐性清创手术前后从健康皮肤和慢性伤口（糖尿病、静脉、动脉或压力）采集拭子样本。我们使用病毒样颗粒富集程序、鸟枪宏基因组测序和基于k聚体的参考依赖性分类学分类方法研究了病毒组。分类组成，多样性，协变量和协会。我们发现，伤口病毒组是高度多样的，许多病毒靶向已知的病原体，并可能影响细菌群落的组成和功能的方式，影响愈合的结果。慢性创伤是一种日益加重的医疗负担。已知这些伤口含有丰富的微生物成分，包括细菌和细菌病毒（细菌病毒）。这些病毒可能在塑造细菌群落结构和功能方面发挥重要作用。我们分析了20例慢性伤口患者的病毒组和细菌组成。与正常皮肤相比，伤口中发现的病毒具有高度多样性，这与细菌组成不同，细菌组成的多样性有所减少。这些数据代表了对慢性伤口微生物组中这一相对未充分研究的组成部分的初步了解，并可能有助于为未来基于噬菌体的干预提供信息。
Two leading impediments to chronic wound healing are polymicrobial infection and biofilm formation. Recent studies have characterized the bacterial fraction of these microbiomes and have begun to elucidate compositional correlations to healing outcomes. However, the factors that drive compositional shifts are still being uncovered. The virome may play an important role in shaping bacterial community structure and function. Previous work on the skin virome determined that it was dominated by bacteriophages, viruses that infect bacteria. To characterize the virome, we enrolled 20 chronic wound patients presenting at an outpatient wound care clinic in a microbiome survey, collecting swab samples from healthy skin and chronic wounds (diabetic, venous, arterial, or pressure) before and after a single, sharp debridement procedure. We investigated the virome using a virus-like particle enrichment procedure, shotgun metagenomic sequencing, and a k-mer-based, reference-dependent taxonomic classification method. Taxonomic composition, diversity, and associations with covariates are presented. We find that the wound virome is highly diverse, with many phages targeting known pathogens, and may influence bacterial community composition and functionality in ways that impact healing outcomes. IMPORTANCE Chronic wounds are an increasing medical burden. These wounds are known to be rich in microbial content, including both bacteria and bacterial viruses (phages). The viruses may play an important role in shaping bacterial community structure and function. We analyzed the virome and bacterial composition of 20 patients with chronic wounds. The viruses found in wounds are highly diverse compared to normal skin, unlike the bacterial composition, where diversity is decreased. These data represent an initial look at this relatively understudied component of the chronic wound microbiome and may help inform future phage-based interventions.
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影响因子：
2.7
作者：
Gardiner M;Vicaretti M;Sparks J;Bansal S;Bush S;Liu M;Darling A;Harry E;Burke CM
通讯作者：
Burke CM
DOI：
10.1073/pnas.1305923110
发表时间：
2013-06-25
影响因子：
11.1
作者：
Barr, Jeremy J.;Auro, Rita;Rohwer, Forest
通讯作者：
Rohwer, Forest
影响因子：
3.2
作者：
Daniel, Anu;Bonnen, Penelope E.;Fischetti, Vincent A.
通讯作者：
Fischetti, Vincent A.
影响因子：
30.3
作者：
Depardieu, Florence;Didier, Jean-Philippe;Bikard, David
通讯作者：
Bikard, David
影响因子：
3.1
作者：
Gjodsbol, Kristine;Skindersoe, Mette E.;Krogfelt, Karen A.
通讯作者：
Krogfelt, Karen A.