Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani.
Assessment of protein silver nanoparticles toxicity against pathogenic Alternaria solani.
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DOI：
10.1007/s13205-016-0515-6
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发表时间：
2016-12
期刊：
影响因子：
2.8
通讯作者：
Mohamed, Mohamed A.
中科院分区：
文献类型：
--
作者：
Abdel-Hafez, Sobhy I. I.;Nafady, Nivien A.;Abdel-Rahim, Ismail R.;Shaltout, Abeer M.;Daros, Jose -Antonio;Mohamed, Mohamed A.
利用内生非致病性早疫病链格孢菌F10（KT 721914）的水提取物进行了银纳米颗粒（AgNPs）的真菌合成。真菌合成的银纳米粒子，其特征在于通过光谱和显微镜技术。在430 nm处发现的表面等离子体共振证实了在室温下几周内形成稳定的AgNP。此外，结果显示形成了平均尺寸为14.8 ± 1.2 nm的球形和单分散的AgNP。FT-IR光谱表明，真菌胞外蛋白和次级代谢产物具有还原Ag和封端AgNPs的作用，形成蛋白Ag纳米复合物。此外，真菌合成的AgNPs对番茄早疫病的病原菌--番茄早疫病链格孢菌的不同致病菌株表现出有效的抗真菌活性。通过测量真菌径向生长的抑制率，在温育8天后评价1、5和10 ppm的AgNP的抗真菌效率。通过扫描和透射电镜观察真菌菌丝形态的变化，进一步支持了上述结果。经处理的真菌菌丝表现出凹坑和孔的形成。此外，真菌合成的AgNP能够通过并分布在整个真菌细胞区域，并与细胞组分相互作用。
Mycogenic synthesis of silver nanoparticles (AgNPs) was carried out in the present investigation using an aqueous extract of endophytic non-pathogenic Alternaria solani F10 (KT721914). The mycosynthesized AgNPs were characterized by means of spectroscopic and microscopic techniques. The surface plasmon resonance found at 430 nm confirmed the formation of stable AgNPs for several weeks at room temperature. Also, the results revealed the formation of spherical and monodispersed AgNPs with an average size of 14.8 ± 1.2 nm. The FT-IR spectrum suggested that the fungal extracellular proteins and secondary metabolites had the role in Ag reduction and AgNPs capping of which protein Ag nanoconjugates were formed. Furthermore, the mycosynthesized AgNPs exhibited potent antifungal activity against different pathogenic isolates of the same Alternaria solani fungus, the causal pathogen of tomato early blight disease. The antifungal efficiency of the AgNPs at 1, 5 and 10 ppm were evaluated for 8 days after incubation by measuring the inhibition rate of fungal radial growth. The results were further supported by investigating fungal hyphae morphology alteration by scanning and transmission electron microscopy. Treated fungal hyphae showed formation of pits and pores. Also, the mycosynthesized AgNPs were able to pass and distribute throughout the fungal cell area and interact with the cell components.
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影响因子：
2.8
作者：
Metuku RP;Pabba S;Burra S;Hima Bindu N SV;Gudikandula K;Singara Charya MA
通讯作者：
Singara Charya MA
DOI：
10.3390/ma6125942
发表时间：
2013-12-18
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
Azizi S;Namvar F;Mahdavi M;Ahmad MB;Mohamad R
通讯作者：
Mohamad R
影响因子：
3.5
作者：
Kim, Keuk-Jun;Sung, Woo Sang;Lee, Dong Gun
通讯作者：
Lee, Dong Gun
DOI：
10.1016/j.nano.2008.04.006
发表时间：
2008-09-01
影响因子：
5.4
作者：
Nadworny, Patricia L.;Wang, JianFei;Burrell, Robert E.
通讯作者：
Burrell, Robert E.
影响因子：
4.4
作者：
Kanmani, Paulraj;Lim, Seung Taik
通讯作者：
Lim, Seung Taik