Bacteria clustering by polymers induces the expression of quorum-sensing-controlled phenotypes.
Bacteria clustering by polymers induces the expression of quorum-sensing-controlled phenotypes.
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细菌利用一系列化学物质来调节其行为并对其环境做出反应。群体感应是细菌利用化学反应来调节感染前行为（如表面附着）的一种手段。因此，可以干扰细菌粘附或用于群体感应的化学反应的聚合物是控制细菌群体反应的潜在手段。在这里，我们报告如何聚合的“细菌螯合剂”，旨在通过静电相互作用结合到细菌，从而抑制细菌粘附到表面，诱导表达的群体感应控制的表型作为细胞聚集的结果。聚合物和分析化学，生物测定和计算建模的组合已被用来模拟细菌聚类和群体感应信号之间的反馈。我们还推导出了在种群水平上控制细菌行为的设计原则和化学策略。
Bacteria deploy a range of chemistries to regulate their behaviour and respond to their environment. Quorum sensing is one mean by which bacteria use chemical reactions to modulate pre-infection behaviour such as surface attachment. Polymers that can interfere with bacterial adhesion or the chemical reactions used for quorum sensing are thus a potential means to control bacterial population responses. Here we report how polymeric “bacteria sequestrants”, designed to bind to bacteria through electrostatic interactions and thus inhibit bacterial adhesion to surfaces, induce the expression of quorum sensing controlled phenotypes as a consequence of cell clustering. A combination of polymer and analytical chemistry, biological assays and computational modelling has been used to characterise the feedback between bacteria clustering and quorum sensing signaling. We have also derived design principles and chemical strategies for controlling bacterial behaviour at the population level.
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影响因子：
15
作者：
Flickinger, Shane T.;Copeland, Matthew F.;Downes, Eric M.;Braasch, Andrew T.;Tuson, Hannah H.;Eun, Ye-Jin;Weibel, Douglas B.
通讯作者：
Weibel, Douglas B.
影响因子：
15
作者：
Garner, Amanda L.;Park, Junguk;Janda, Kim D.
通讯作者：
Janda, Kim D.
影响因子：
14.8
作者：
Carnes EC;Lopez DM;Donegan NP;Cheung A;Gresham H;Timmins GS;Brinker CJ
通讯作者：
Brinker CJ
影响因子：
38.3
作者：
Liu, Lihong;Xu, Kaijin;Yang, Yi-Yan
通讯作者：
Yang, Yi-Yan
影响因子：
--
作者：
Diggle, Stephen P.;Matthijs, Sandra;Williams, Paul
通讯作者：
Williams, Paul